从腹泻患者中分离出的豚鼠气单胞菌，其喹诺酮耐药决定区的II型拓扑异构酶突变与异常高水平的喹诺酮耐药性相关。
An unusually high level of quinolone resistance associated with type II topoisomerase mutations in quinolone resistance-determining regions of Aeromonas caviae isolated from diarrhoeal patients.
作者信息
Sinha Sutapa, Chattopadhyay S, Bhattacharya S K, Nair G B, Ramamurthy T
机构信息
National Institute of Cholera and Enteric Diseases, Beliaghata, Kolkata 700 010, India.
出版信息
Res Microbiol. 2004 Dec;155(10):827-9. doi: 10.1016/j.resmic.2004.06.008.
We examined 158 strains belonging to different Aeromonas species isolated from hospitalized acute diarrhoea cases for susceptibility to quinolones. Compared to other species, a high percentage of the A. caviae strains were resistant to ciprofloxacin and norfloxacin. Based on MIC values, 6 A. caviae strains were selected and the nucleotide sequences for the quinolone-resistant-determining regions (QRDRs) of gyrA, gyrB and parC genes were analysed. In resistant strains, double mutations (Ser(83)-->Ile and Asp(87)-->Asn) and a single mutation (Ser(80)-->Ile) were detected in the QRDR of gyrA and parC, respectively.
我们检测了从住院急性腹泻病例中分离出的属于不同气单胞菌属的158株菌株对喹诺酮类药物的敏感性。与其他菌种相比，豚鼠气单胞菌菌株对环丙沙星和诺氟沙星的耐药率较高。根据最低抑菌浓度（MIC）值，选择了6株豚鼠气单胞菌菌株，并分析了gyrA、gyrB和parC基因喹诺酮耐药决定区（QRDR）的核苷酸序列。在耐药菌株中，分别在gyrA和parC的QRDR中检测到双突变（Ser(83)-->Ile和Asp(87)-->Asn）和单突变（Ser(80)-->Ile）。
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