Sinico Chiara, De Logu Alessandro, Lai Francesco, Valenti Donatella, Manconi Maria, Loy Giuseppe, Bonsignore Leonardo, Fadda Anna Maria
Dipartimento Farmaco Chimico Tecnologico, Università di Cagliari, Cagliari, Italy.
Eur J Pharm Biopharm. 2005 Jan;59(1):161-8. doi: 10.1016/j.ejpb.2004.06.005.
The effect of liposomal inclusion on the in vitro antiherpetic activity of Artemisia arborescens L. essential oil was investigated. In order to study the influence of vesicle structure and composition on the antiviral activity of the vesicle-incorporated oil, multilamellar (MLV) and unilamellar (SUV) positively charged liposomes were prepared by the film method and sonication. Liposomes were obtained from hydrogenated (P90H) and non-hydrogenated (P90) soy phosphatidylcholine. Formulations were examined for their stability for over one year, monitoring the oil leakage from vesicles and the average size distribution. The antiviral activity was studied against Herpes simplex virus type 1 (HSV-1) by a quantitative tetrazolium-based colorimetric method. Results showed that Artemisia essential oil can be incorporated in good amounts in the prepared vesicular dispersions. Stability studies pointed out that vesicle dispersions were very stable for at least six months and neither oil leakage nor vesicle size alteration occurred during this period. After one year of storage oil retention was still good, but vesicle fusion was present. Antiviral assays demonstrated that the liposomal incorporation of A. arborescens essential oil enhanced its in vitro antiherpetic activity especially when vesicles were made with P90H. On the contrary, no significant difference in antiviral activity was observed between the free and SUV-incorporated oil.
研究了脂质体包封对树蒿艾精油体外抗疱疹活性的影响。为了研究囊泡结构和组成对囊泡包封油抗病毒活性的影响，采用薄膜法和超声法制备了多层（MLV）和单层（SUV）带正电荷的脂质体。脂质体由氢化（P90H）和非氢化（P90）大豆磷脂酰胆碱制成。对制剂进行了一年多的稳定性检测，监测油从囊泡中的泄漏情况和平均粒径分布。采用基于四唑的定量比色法研究了对1型单纯疱疹病毒（HSV-1）的抗病毒活性。结果表明，树蒿艾精油能大量包封于所制备的囊泡分散体中。稳定性研究指出，囊泡分散体至少六个月内非常稳定，在此期间既没有油泄漏也没有囊泡大小改变。储存一年后，油保留率仍然良好，但存在囊泡融合现象。抗病毒试验表明，树蒿艾精油的脂质体包封增强了其体外抗疱疹活性，尤其是当用P90H制备囊泡时。相反，游离油和SUV包封油之间在抗病毒活性上没有观察到显著差异。
