Tomimura Kenta, Spak Josef, Katis Nikos, Jenner Carol E, Walsh John A, Gibbs Adrian J, Ohshima Kazusato
Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan.
Virology. 2004 Dec 20;330(2):408-23. doi: 10.1016/j.virol.2004.09.040.
The genetic structure of populations of Turnip mosaic virus in Eurasia was assessed by making host range and gene sequence comparisons of 142 isolates. Most isolates collected in West Eurasia infected Brassica plants whereas those from East Eurasia infected both Brassica and Raphanus plants. Analyses of recombination sites (RSs) in five regions of the genome (one third of the full sequence) showed that the protein 1 (P1 gene) had recombined more frequently than the other gene regions in both subpopulations, but that the RSs were located in different parts of the genomes of the subpopulations. Estimates of nucleotide diversity showed that the West Eurasian subpopulation was more diverse than the East Eurasian subpopulation, but the Asian-BR group of the genes from the latter subpopulation had a greater nonsynonymous/synonymous substitution ratio, especially in the P1, viral genome-linked protein (VPg) and nuclear inclusion a proteinase (NIa-Pro) genes. These subpopulations seem to have evolved independently from the ancestral European population, and their genetic structure probably reflects founder effects.
通过对142个芜菁花叶病毒分离株进行宿主范围和基因序列比较，评估了欧亚大陆芜菁花叶病毒种群的遗传结构。在欧亚大陆西部收集的大多数分离株感染芸苔属植物，而来自欧亚大陆东部的分离株则感染芸苔属和萝卜属植物。对基因组五个区域（全序列的三分之一）的重组位点（RSs）分析表明，在两个亚种群中，蛋白质1（P1基因）的重组频率均高于其他基因区域，但RSs位于亚种群基因组的不同部位。核苷酸多样性估计表明，欧亚大陆西部亚种群比欧亚大陆东部亚种群更加多样化，但后一个亚种群的亚洲 - BR基因群具有更高的非同义/同义替换率，尤其是在P1、病毒基因组连接蛋白（VPg）和核内含物蛋白酶（NIa-Pro）基因中。这些亚种群似乎是从欧洲祖先种群独立进化而来的，它们的遗传结构可能反映了奠基者效应。
