Laina Jojo Armando, Matsumoto Kosuke, Setoyama Tamotsu, Kawano Shinji, Ohshima Kazusato
Laboratory of Plant Virology, Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, 1-banchi, Honjo-machi, Saga, 840-8502, Japan.
Pharmaceutical Business Division, Yakult Honsha CO., LTD., 16-21 Ginza 7-chome, Chuo-ku, Tokyo, 104-0061, Japan.
Virus Genes. 2019 Jun;55(3):347-355. doi: 10.1007/s11262-019-01656-0. Epub 2019 Mar 20.
Pepper veinal mottle virus (PVMV) is known to infect chilli pepper and belongs to the Chilli veinal mottle virus phylogroup of potyviruses. PVMV has recently appeared in Japan. In this study, we report six complete genomic sequences of PVMV isolates from chilli pepper (i.e. Capsicum annuum) in Okinawa Islands in Japan, and we determined the evolutionary relationships between Japanese isolates and the isolates reported earlier from African and Asian countries. Complete genomic sequences of the six Japanese PVMV isolates were 9760 nucleotides in length, excluding the nucleotide primer sequences used for amplifying 5' end of the genomes. The major findings of this study are as follows: (1) all the Japanese isolates of PVMV have similar biological and molecular characteristics, indicating the presence of only one population in Japan; (2) there are at least three major phylogenetic groups of PVMV worldwide; (3) PVMV probably originated in East Africa; and (4) all the Asian isolates are closely related to the Ghanaian isolate.
辣椒脉斑驳病毒（PVMV）已知可感染辣椒，属于马铃薯Y病毒属辣椒脉斑驳病毒组。PVMV最近在日本出现。在本研究中，我们报告了来自日本冲绳群岛辣椒（即辣椒属）的6个PVMV分离株的完整基因组序列，并确定了日本分离株与先前报道的来自非洲和亚洲国家的分离株之间的进化关系。6个日本PVMV分离株的完整基因组序列长度为9760个核苷酸，不包括用于扩增基因组5'端的核苷酸引物序列。本研究的主要发现如下：（1）所有日本PVMV分离株具有相似的生物学和分子特征，表明日本仅存在一个种群；（2）全球PVMV至少有三个主要系统发育组；（3）PVMV可能起源于东非；（4）所有亚洲分离株与加纳分离株密切相关。
