Kageyama Chizuko, Ohta Toshiya, Hiraoka Kazuyo, Suzuki Morimasa, Okamoto Tetsuji, Ohishi Kazuo
Numazu Industrial Research Institute of Shizuoka Prefecture, Ohoka, Numazu, Shizuoka 410-0022, Japan.
Arch Microbiol. 2005 Jan;183(1):56-65. doi: 10.1007/s00203-004-0746-5. Epub 2004 Nov 27.
Two strains of trichloroethylene (TCE)-degrading bacteria were isolated from soils at polluted and unpolluted sites. The isolates, strains TE26(T) and K6, showed co-substrate-independent TCE-degrading activity. TCE degradation was accelerated by preincubation with tetrachloroethylene, cis-dichloroethylene (DCE) and 1,1-DCE. TCE-degrading activities of strains TE26(T) and K6 were 0.23, 0.24 micromol min(-1) g(-1) dry cells, respectively. 16S rDNA sequences of strains TE26(T) and K6 were almost identical (99.7% similarity), and most closely related to Ralstonia basilensis (ATCC17697(T)) (98.5% similarity). From the results of DNA-DNA hybridizations, strain TE26(T) was genetically coherent to strain K6 (94 and 88% hybridization), and exhibited lower relatedness to R. basilensis (DSM11853(T)) (44% and 15%). In addition, because of the differences in chemotaxonomic properties, strain TE26(T) and strain K6 appear to be distinct from all established species of the Ralstonia group. Based on these results and the proposal of transferring R. basilensis and related species to Wautersia gen. nov., we propose that these strains should be assigned to the genus Wautersia as Wautersia numadzuensis sp. nov.
从污染和未污染场地的土壤中分离出两株三氯乙烯（TCE）降解菌。分离菌株TE26(T)和K6表现出不依赖共底物的TCE降解活性。用四氯乙烯、顺式二氯乙烯（DCE）和1,1-二氯乙烯预培养可加速TCE的降解。菌株TE26(T)和K6的TCE降解活性分别为0.23、0.24 μmol min(-1) g(-1)干细胞。菌株TE26(T)和K6的16S rDNA序列几乎相同（相似度99.7%），与罗尔斯通氏菌（Ralstonia basilensis）（ATCC17697(T)）关系最为密切（相似度98.5%）。根据DNA-DNA杂交结果，菌株TE26(T)与菌株K6在基因上具有一致性（杂交率94%和88%），与罗尔斯通氏菌（R. basilensis）（DSM11853(T)）的相关性较低（44%和15%）。此外，由于化学分类学特性的差异，菌株TE26(T)和菌株K6似乎与罗尔斯通氏菌属所有已确定的物种都不同。基于这些结果以及将罗尔斯通氏菌和相关物种转移到沃氏菌属（Wautersia gen. nov.）的提议，我们建议将这些菌株归为沃氏菌属，命名为新种Numazu沃氏菌（Wautersia numadzuensis sp. nov.）。
