Appenzeller Laura M, Munley Susan M, Hoban Denise, Sykes Greg P, Malley Linda A, Delaney Bryan
Pioneer Hi-Bred International, Inc., Johnston, IA, USA.
Food Chem Toxicol. 2008 Jun;46(6):2201-13. doi: 10.1016/j.fct.2008.02.017. Epub 2008 Feb 29.
Optimum GAT1 soybean is a genetically modified (GM) soybean containing event DP-356Ø43-5 (356043) that was produced by integration of the coding sequences of the GAT4601 and GM-HRA proteins. In planta expression of these proteins confers tolerance to glyphosate and sulfonylurea/imidazolinone herbicides, respectively. This paper reports the results from a subchronic rat feeding study conducted with 356043 soybeans. Dehulled/defatted toasted meal and toasted ground hulls were prepared from soybeans from untreated plants (356043), herbicide-treated plants (356043+Gly/SU), non-transgenic isoline control (091), and three commercial non-transgenic reference varieties (93B86, 93B15, and 93M40). Individual diets conforming to standard certified rodent chow formulation (Purina Rodent LabDiet) 5002) were prepared with 20% meal (w/w) and 1.5% hulls (w/w). Diets were fed to young adult Sprague-Dawley rats (12/sex/group) for at least 93 days. Compared with rats fed the isoline control or conventional reference diets, no biologically-relevant, adverse effects were observed in rats fed diets containing 356043 or 356043+Gly/SU soybean with respect to body weight/gain, food consumption/efficiency, clinical signs, mortality, ophthalmology, neurobehavioral assessments (sensory response, grip strength, motor activity), clinical pathology (hematology, coagulation, serum chemistry, urinalysis), organ weights, and gross and microscopic pathology. The results from this study indicate that 356043 soybeans are as safe and nutritious as conventional non-GM soybeans.
最优GAT1大豆是一种转基因大豆，含有事件DP - 356043 - 5（356043），它通过整合GAT4601和GM - HRA蛋白的编码序列产生。这些蛋白在植物中的表达分别赋予了对草甘膦和磺酰脲/咪唑啉酮类除草剂的耐受性。本文报告了用356043大豆进行的亚慢性大鼠喂养研究的结果。从未经处理的植株（356043）、经除草剂处理的植株（356043 + Gly/SU）、非转基因近等基因系对照（091）以及三个商业非转基因参考品种（93B86、93B15和93M40）的大豆中制备了去皮/脱脂烤粕和烤碎豆皮。按照标准认证的啮齿动物饲料配方（普瑞纳啮齿动物实验室饲料5002），用20%的粕（重量/重量）和1.5%的豆皮（重量/重量）制备了个体日粮。将日粮喂给年轻成年斯普拉格 - 道利大鼠（每组12只/性别）至少93天。与喂食近等基因系对照或传统参考日粮的大鼠相比，在喂食含有356043或356043 + Gly/SU大豆日粮的大鼠中，未观察到与体重/增重、食物消耗/效率、临床症状、死亡率、眼科、神经行为评估（感觉反应、握力、运动活动）、临床病理学（血液学、凝血、血清化学、尿液分析）、器官重量以及大体和显微镜病理学相关的生物学上有意义的不良影响。本研究结果表明，356043大豆与传统非转基因大豆一样安全且营养丰富。
