[扁蓄豆对1型单纯疱疹病毒体外抗病毒机制的实验研究]

[Experimental study on the antiviral mechanism of Ceratostigma willmattianum against herpes simplex virus type 1 in vitro].

作者信息

Chen Tian, Jia Wen-xiang, Yang Fa-long, Xie Yi, Yang Wei-qing, Zeng Wei, Zhang Zai-rong, Li Hui, Jiang Si-ping, Yang Zhen, Chen Jin-rui

机构信息

Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041,China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2004 Sep;29(9):882-6.

PMID:15575210

Abstract

OBJECTIVE

To study the antiviral effect and mechanisms of the liquid extract from Ceratostigma willmattianum against herpes simplex virus type 1 (HSV-1) in vitro.

METHOD

C. willmattianum in various concentration was applied to different steps of HSV-1 replication cycle. 50% Tissue culture infective dose (TCID50), cytopathic effect (CPE), MTT staining method, dot blotting and Northern blotting analysis were used to estimate index of antiviral activity.

RESULT

50% Toxic concentration (TC50) was 1077 mg x L(-1), IC50 29.46 mg x L(-1) and therapeutic index (TI) 36.56 in C. willmattianum. TC50 330 mg x L(-1), 50% Inhibiting concentration (IC50) 9.12 mg x L(-1) and TI 36.18 in ACV by MTT staining method. The liquid extract from C. willmattianum had remarkable effect on inhibiting HSV-1 in vitro. Ceratostigma could interfere absorption of HSV-1 to Vero cells to prevent HSV-1 infectivity, inhibit HSV-1 gD DNA replication and HSV-1 gD mRNA expression.

CONCLUSION

C. willmattianum possesses strong anti-HSV-1 activity in vitro. The antiviral mechanisms are related to inhibiting virus absorption, HSV-1 gD gene replication and HSV-1 gD gene transcription.

摘要

目的

研究西南紫薇液体提取物对1型单纯疱疹病毒（HSV-1）的体外抗病毒作用及机制。

方法

将不同浓度的西南紫薇应用于HSV-1复制周期的不同阶段。采用50%组织培养感染剂量（TCID50）、细胞病变效应（CPE）、MTT染色法、斑点杂交和Northern杂交分析来评估抗病毒活性指标。

结果

西南紫薇的50%毒性浓度（TC50）为1077mg·L⁻¹，半数抑制浓度（IC50）为29.46mg·L⁻¹，治疗指数（TI）为36.56。通过MTT染色法测得阿昔洛韦的TC50为330mg·L⁻¹，IC50为9.12mg·L⁻¹，TI为36.18。西南紫薇液体提取物在体外对HSV-1具有显著的抑制作用。西南紫薇可干扰HSV-1对Vero细胞的吸附以防止HSV-1感染性，抑制HSV-1 gD DNA复制和HSV-1 gD mRNA表达。