Clark Melanee D, Krugner-Higby Lisa, Smith Lesley J, Heath Timothy D, Clark Krystal L, Olson Dawne
Laboratory Animal Services, 414 E. Clark St., Lee Medical Bldg., University of South Dakota, Vermillion, South Dakota 57069, USA.
Comp Med. 2004 Oct;54(5):558-63.
The use of mice in biomedical research is increasing, largely due to the production and use of genetically engineered animals. Providing postoperative pain control in mice presents many challenges, and long-acting analgesic preparations would be advantageous for this species. A single subcutaneous injection of a liposome-encapsulated (LE) preparation of oxymorphone was compared with multiple injections of buprenorphine or saline in outbred mice undergoing splenectomy. Control groups were given isoflurane alone or isoflurane and an injection of LE oxymorphone but did not undergo surgery. The following parameters were evaluated for 5 days after surgery and were compared with presurgical baseline data for each group: food and water consumption, body weight, ethographic score, and voluntary exercise on a running wheel. Ethographic scores indicated less postsurgical pain in both groups of mice that received either analgesic preparation compared with mice that received only saline. However, mice given LE oxymorphone had superior postoperative recovery, as measured by wheel-running distance and body weight gain, compared with mice given buprenorphine or saline. Mice undergoing splenectomy had significant decreases in body weight, food and water consumption, voluntary exercise, and other normal behaviors. Administration of liposomal oxymorphone at the time of surgery improved postsurgical recovery as measured by these parameters compared with multiple injections of buprenorphine or saline alone. Administration of LE oxymorphone at the time of surgery improved postsurgical recovery, as measured by these parameters.
在生物医学研究中，小鼠的使用量正在增加，这主要归因于基因工程动物的培育和使用。为小鼠提供术后疼痛控制面临诸多挑战，长效镇痛制剂对该物种将具有优势。将单次皮下注射脂质体包裹（LE）的羟吗啡酮制剂与对接受脾切除术的远交系小鼠多次注射丁丙诺啡或生理盐水进行了比较。对照组仅给予异氟烷或异氟烷并注射LE羟吗啡酮，但不进行手术。术后5天对以下参数进行评估，并与每组术前基线数据进行比较：食物和水的消耗量、体重、行为学评分以及在跑步轮上的自主运动量。行为学评分表明，与仅接受生理盐水的小鼠相比，接受任何一种镇痛制剂的两组小鼠术后疼痛较轻。然而，与给予丁丙诺啡或生理盐水的小鼠相比，给予LE羟吗啡酮的小鼠术后恢复情况更佳，这通过跑步距离和体重增加来衡量。接受脾切除术的小鼠体重、食物和水的消耗量、自主运动量以及其他正常行为均显著下降。与多次注射丁丙诺啡或单独注射生理盐水相比，手术时给予脂质体羟吗啡酮可改善这些参数所衡量的术后恢复情况。手术时给予LE羟吗啡酮可改善这些参数所衡量的术后恢复情况。
