Goldkuhl R, Carlsson H-E, Hau J, Abelson K S P
Division of Comparative Medicine, Department of Neuroscience, Uppsala University, Uppsala, Sweden.
Eur Surg Res. 2008;41(3):272-8. doi: 10.1159/000142372. Epub 2008 Jul 3.
Adequate peri-operative analgesia may reduce post-operative stress response and improve recovery in laboratory animals. We have established a method involving repeated automated blood sampling, allowing quantification of serum corticosterone levels in rats for stress assessment without stress-inducing handling or restraint. In the present study, the effects of the commonly used route of buprenorphine administration (0.05 mg/kg injected subcutaneously) were compared with oral administration (0.4 mg/kg mixed with Nutella and orally administered by voluntary ingestion) in male Sprague-Dawley rats.
A catheter was placed in the jugular vein and attached to an Accusampler for automated blood sampling. During 96 h after surgery, blood was collected at specified time points. Pre- and post-operative body weights and water consumption were registered.
Buprenorphine significantly suppressed levels of circulating corticosterone after the oral but not after the subcutaneous treatment. Both buprenorphine treatments had a positive impact on maintenance of body weight and water consumption, compared to the control group that received no buprenorphine.
The present investigation suggests that oral voluntary ingestion ad libitum is an efficacious, convenient and non-invasive way of administering peri-operative buprenorphine to rats, as judged by corticosteroid response and effects on body weight and water consumption.
充分的围手术期镇痛可能会减轻实验动物的术后应激反应并促进恢复。我们建立了一种涉及重复自动采血的方法，可在不进行应激诱导处理或约束的情况下，对大鼠血清皮质酮水平进行定量以评估应激。在本研究中，比较了常用的丁丙诺啡给药途径（皮下注射0.05mg/kg）与口服给药（与能多益混合，以0.4mg/kg的剂量经自愿摄入口服）对雄性Sprague-Dawley大鼠的影响。
将一根导管置于颈静脉，并连接到Accusampler进行自动采血。在手术后的96小时内，在特定时间点采集血液。记录术前和术后的体重及饮水量。
丁丙诺啡口服给药后可显著抑制循环皮质酮水平，而皮下给药则无此效果。与未接受丁丙诺啡的对照组相比，两种丁丙诺啡给药方式对体重维持和饮水量均有积极影响。
根据皮质类固醇反应以及对体重和饮水量的影响判断，本研究表明，大鼠围手术期口服丁丙诺啡是一种有效、方便且无创的给药方式。
