Fujimaki Y, Sithithaworn P, Mitsui Y, Aoki Y
Department of Parasitology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, 852-8523 Japan.
J Helminthol. 2004 Dec;78(4):293-5. doi: 10.1079/joh2004263.
The macrofilaricidal activity of diethylcarbamazine (DEC) was confirmed in jirds infected with Brugia pahangi. Seventy jirds were inoculated subcutaneously with 100 infective larvae. At 20 weeks post-infection, the microfilaraemic jirds were divided into two groups, untreated and treated. For the treated group, 200 mg kg(-1) of DEC was injected intraperitoneally for 5 consecutive days. One, 4, 8, 12, 16 and 27 weeks after the final treatment, 4-7 jirds in each group were sacrificed to measure adult worm burdens. The number of adult worms recovered from treated jirds was comparable to controls at earlier necropsy (1 and 4 weeks post-treatment). However, at late necropsy (8 weeks and later) the recovery rate of adult worms in treated jirds was significantly lower than that in untreated controls, indicating an adultcidal effect of DEC. The present study demonstrates that DEC requires 8 weeks to kill B. pahangi adult worms in jirds and that the Mongolian jird is a useful model for screening antifilarial activity.
乙胺嗪（DEC）对感染彭亨布鲁线虫的沙鼠的杀成虫活性得到了证实。70只沙鼠皮下接种100条感染性幼虫。感染后20周，将出现微丝蚴血症的沙鼠分为两组，一组不治疗，另一组治疗。治疗组连续5天腹腔注射200mg/kg的DEC。最后一次治疗后1、4、8、12、16和27周，每组处死4 - 7只沙鼠以测量成虫负荷。在早期尸检（治疗后1周和4周）时，从治疗沙鼠体内回收的成虫数量与对照组相当。然而，在晚期尸检（8周及以后）时，治疗沙鼠体内成虫的回收率显著低于未治疗的对照组，表明DEC具有杀成虫作用。本研究表明，DEC需要8周时间才能杀死沙鼠体内的彭亨布鲁线虫成虫，并且蒙古沙鼠是筛选抗丝虫活性的有用模型。
