Mayanagi M, Nakayama S, Oguchi K
Department of Pharmacology, School of Medicine, Showa University, Tokyo, Japan.
Nihon Yakurigaku Zasshi. 1992 Feb;99(2):115-21. doi: 10.1254/fpj.99.115.
Eight kinds of hot water extracts (HWE) and 2 kinds of distanninized fractions (DTF) from Umbelliferae herbs were prepared. The effects of HWE and DTF on rat hepatic lipid peroxidation (LPO), aminopyrine N-demethylase (APD) and aniline hydroxylase (ANH) activities were examined in vitro. The APD activity was inhibited by HWE, DTF from Byakushi and HWE from Uikyo, Zenko, Toki, Senkyu, Bofu and Saiko, respectively, whereas DTF from Uikyo caused no significant effect. The ANH activity was inhibited by HWE, DTF from Byakushi, Uikyo and HWE from Bofu, Zenko, Hokushajin and Toki, respectively. LPO was inhibited by HWE from Zenko, Byakushi and Senkyu, but increased by Saiko, Uikyo, Bofu and Hokushajin. The results of Uikyo DTF suggests that the components of Uikyo that altered the activity of APD and ANH are different respectively. Marked inhibitions on the activity of APD and ANH caused by Byakushi indicate that Byakushi might affect the activity of hepatic drug metabolizing enzymes in vivo.
制备了八种伞形科草药的热水提取物（HWE）和两种水飞蓟宾化馏分（DTF）。体外研究了HWE和DTF对大鼠肝脏脂质过氧化（LPO）、氨基比林N - 脱甲基酶（APD）和苯胺羟化酶（ANH）活性的影响。APD活性分别被HWE、白蔹的DTF以及鬼臼、前胡、当归属、川芎、防风和柴胡的HWE抑制，而鬼臼的DTF未产生显著影响。ANH活性分别被HWE、白蔹的DTF、鬼臼的DTF以及防风、前胡、北沙参和当归属的HWE抑制。LPO被前胡、白蔹和川芎的HWE抑制，但被柴胡、鬼臼、防风和北沙参升高。鬼臼DTF的结果表明，鬼臼中改变APD和ANH活性的成分各不相同。白蔹对APD和ANH活性的显著抑制表明，白蔹可能在体内影响肝脏药物代谢酶的活性。
