Raj J U, Anderson J
Department of Pediatrics, Harbor-UCLA Medical Center, University of California Los Angeles School of Medicine, Torrance 90509.
J Appl Physiol (1985). 1992 Feb;72(2):699-705. doi: 10.1152/jappl.1992.72.2.699.
We have determined regional lung interstitial fluid albumin concentration in lambs with hydrostatic pulmonary edema and correlated it with lung lymph and plasma albumin concentrations. In anesthetized lambs, we raised left atrial pressure to 25-30 cmH2O by obstructing the aorta and volume overloading the lambs with infusions of Ringer lactate solution (group I, n = 10) or sheep's blood (group II, n = 9). We measured lung lymph flow and concentrations of total protein and albumin in plasma and lymph. With micropipettes we also collected interstitial fluid from interlobular septal pools and peribronchial, periarterial, and perivenous liquid cuffs near the hilum for measurement of albumin concentration by the gel immunoelectrophoresis method. In both groups, lung lymph flow increased with left atrial hypertension, and the ratio of lymph to plasma protein concentration fell. For group I, plasma and lymph albumin concentrations during the phase of hydrostatic edema were 1.97 +/- 0.49 and 1.15 +/- 0.36, respectively; for group II, they were 3.77 +/- 0.42 and 2.43 +/- 0.39 g/dl, respectively. Lung wet-to-dry weight ratio averaged 6.0 in both groups. Albumin concentration was always lower in interstitial fluid than in plasma. In both groups, albumin concentration was similar in periarterial and peribronchial fluid cuffs (group I 1.19 +/- 0.6 and 1.36 +/- 0.79 g/dl, respectively; group II 2.87 +/- 1.05 and 2.33 +/- 0.58 g/dl, respectively) but was always greater than that in perivenous and interlobular septal pools (group I 0.61 +/- 0.21 and 0.67 +/- 0.23 g/dl, respectively; group II 1.76 +/- 0.49 and 1.55 +/- 0.52 g/dl, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
我们测定了患有静水压性肺水肿的羔羊肺间质液白蛋白浓度，并将其与肺淋巴液和血浆白蛋白浓度进行了关联。在麻醉的羔羊中，我们通过阻塞主动脉并向羔羊输注乳酸林格液（第一组，n = 10）或羊血（第二组，n = 9）使其左心房压力升高至25 - 30 cmH₂O。我们测量了肺淋巴液流量以及血浆和淋巴液中总蛋白和白蛋白的浓度。我们还用微量移液器从肺门附近的小叶间隔池以及支气管周围、动脉周围和静脉周围的液体袖套中收集间质液，通过凝胶免疫电泳法测量白蛋白浓度。在两组中，肺淋巴液流量随左心房高压而增加，淋巴液与血浆蛋白浓度之比下降。对于第一组，静水压性水肿阶段的血浆和淋巴液白蛋白浓度分别为1.97±0.49和1.15±0.36；对于第二组，分别为3.77±0.42和2.43±0.39 g/dl。两组的肺湿重与干重之比平均为6.0。间质液中的白蛋白浓度始终低于血浆中的浓度。在两组中，动脉周围和支气管周围液体袖套中的白蛋白浓度相似（第一组分别为1.19±0.6和1.36±0.79 g/dl；第二组分别为2.87±1.05和2.33±0.58 g/dl），但始终高于静脉周围和小叶间隔池中的浓度（第一组分别为0.61±0.21和0.67±0.23 g/dl；第二组分别为1.76±0.49和1.55±0.52 g/dl）。（摘要截取自250字）
