Acheré V, Favre J M, Besnard G, Jeandroz S
UMR INRA/UHP 1136: Tree-microbe Interactions, Faculté des Sciences, Université Henri Poincaré-Nancy 1 BP 239, F-54506 Vandoeuvre-lès-Nancy, France.
Mol Ecol. 2005 Sep;14(10):3191-201. doi: 10.1111/j.1365-294X.2005.02646.x.
Diversity and differentiation among three populations representing the geographical domains commonly recognized within the natural distribution area of Picea abies were analysed by using a set of 292 AFLP (amplified fragment length polymorphism), SSR (single sequence repeat) and ESTP (expressed sequence tags polymorphism) markers. As usually observed in forest trees, results showed high within-population diversity (H(S) reaching 0.79) and low among-population differentiation (G(ST) approximately 2%). The genomic organization of differentiation was then investigated on the basis of a subsample of 150 AFLP, SSR and ESTP mapped markers. The number of the loci differentiating the Baltico-Nordic from the central European populations (25 loci) and, within the central European populations, the Alpine from the Hercyno-Carpathian populations (12 loci), were different. These 37 differentiated loci, with individual G(ST) values ranging from 0.008 to 0.20, were evenly distributed on all linkage groups and mostly followed the neutral expectations, suggesting genome-wide effects on differentiation. Nine of them however behave as 'outlier' loci indicating possible locus-specific selective effects. Contribution of ongoing evolutionary forces and historical effects to the geographical differentiation of the species are discussed.
利用一组292个AFLP（扩增片段长度多态性）、SSR（单序列重复）和ESTP（表达序列标签多态性）标记，分析了代表欧洲云杉自然分布区内通常认可的地理区域的三个种群之间的多样性和分化情况。正如在林木中通常观察到的那样，结果显示种群内多样性较高（H(S)达到0.79），而种群间分化较低（G(ST)约为2%）。然后基于150个AFLP、SSR和ESTP定位标记的子样本研究了分化的基因组组织。区分波罗的海 - 北欧种群与中欧种群的基因座数量（25个基因座），以及在中欧种群中，区分阿尔卑斯种群与海西 - 喀尔巴阡种群的基因座数量（12个基因座）是不同的。这37个分化基因座，其个体G(ST)值范围从0.008到0.20，均匀分布在所有连锁群上，并且大多符合中性预期，表明对分化存在全基因组效应。然而，其中9个表现为“异常”基因座，表明可能存在基因座特异性选择效应。文中讨论了正在进行的进化力量和历史效应对该物种地理分化的贡献。
