Qi Lu, Shen Haiqing, Larson Ilona, Schaefer Ernst J, Greenberg Andrew S, Tregouet David A, Corella Dolores, Ordovas Jose M
Nutrition and Genomics Laboratories, Jean Mayer-U.S. Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA.
Obes Res. 2004 Nov;12(11):1758-65. doi: 10.1038/oby.2004.218.
Perilipin is a class of protein-coating lipid droplets in adipocytes and steroidogenic cells. Our purpose was to examine the association between common single-nucleotide polymorphisms (SNPs) at the perilipin (PLIN) locus and obesity, as well as related phenotypes, in unrelated American adults.
Four PLIN SNPs (PLIN 6209T>C, 11482G>A, 13041A>G, and 14995A>T) were typed in 734 white subjects (373 men and 361 women) attending a residential lifestyle intervention program. The baseline anthropometric and biochemical measures were used. Obesity was defined as BMI > or = 30 kg/m(2).
Multivariate analysis demonstrated that, in women, two of the SNPs (13041A>G, and 14995A>T) were significantly associated with percentage body fat (p = 0.016 for 13041A>G and p = 0.010 for 14995A>T) and waist circumference (p = 0.020 for 13041A>G and p = 0.045 for 14995A>T). Moreover, haplotype analysis using these two SNPs indicated that haplotypes A/T and G/T were both associated with significantly increased obesity risk (odds ratio = 1.76, 95% confidence interval 1.07 to 2.90 for haplotype A/T, and odds ratio = 1.73, 95% confidence interval 1.06 to 2.82 for haplotype G/T) when compared with haplotype A/A. No significant associations between PLIN variations and obesity were found in men.
Our data support the hypothesis that the PLIN locus may be a significant genetic determinant for obesity risk in whites and that women are more sensitive to the genetic effects of perilipin than men.
围脂滴蛋白是一类存在于脂肪细胞和类固醇生成细胞中包裹脂质小滴的蛋白质。我们的目的是研究无关美国成年人中围脂滴蛋白（PLIN）基因座常见单核苷酸多态性（SNP）与肥胖及相关表型之间的关联。
对参加居家生活方式干预项目的734名白人受试者（373名男性和361名女性）进行4种PLIN SNP（PLIN 6209T>C、11482G>A、13041A>G和14995A>T）分型。采用基线人体测量和生化指标。肥胖定义为体重指数（BMI）≥30 kg/m²。
多变量分析表明，在女性中，其中两个SNP（13041A>G和14995A>T）与体脂百分比显著相关（13041A>G的p = 0.016，14995A>T的p = 0.010）以及腰围（13041A>G的p = 0.020，14995A>T的p = 0.045）。此外，使用这两个SNP进行单倍型分析表明，与单倍型A/A相比，单倍型A/T和G/T均与肥胖风险显著增加相关（单倍型A/T的优势比 = 1.76，95%置信区间1.07至2.90；单倍型G/T的优势比 = 1.73，95%置信区间1.06至2.82）。在男性中未发现PLIN变异与肥胖之间存在显著关联。
我们的数据支持以下假设：PLIN基因座可能是白人肥胖风险的一个重要遗传决定因素，并且女性比男性对围脂滴蛋白的遗传效应更敏感。
