Lipid Research Laboratory, IIS-Fundación Jiménez Díaz, 28040 Madrid, Spain.
Laboratory of Diabetes and Vascular Pathology, IIS-Fundación Jiménez Díaz, UAM, 28040 Madrid, Spain.
Nutrients. 2022 Jul 26;14(15):3072. doi: 10.3390/nu14153072.
Variations in the perilipin () gene have been suggested to be associated with obesity and its related alterations, but a different nutritional status seems to contribute to differences in these associations. In our study, we examined the association of several polymorphisms at the locus with obesity and lipid profile in children, and then analyzed the mediation of plasma leptin levels on these associations. The single-nucleotide polymorphisms (SNPs) rs894160, rs1052700, and rs2304795 in , and rs35568725 in , were analyzed by RT-PCR in 1264 children aged 6-8 years. Our results showed a contrasting association of rs1052700 with apolipoprotein (Apo) A-I levels in boys and girls, with genotype TT carriers showing significantly higher Apo A-I levels in boys and significantly lower Apo A-I levels in girls. Significant associations of the SNP rs35568725 with high-density lipoprotein cholesterol (HDL-cholesterol), Apo A-I, and non-esterified fatty acids (NEFA) were observed in boys but not in girls. The associations of the SNPs studied with body mass index (BMI), NEFA, and Apo A-I in boys and girls were different depending on leptin concentration. In conclusion, we describe the mediation of plasma leptin levels in the association of SNPs in and with BMI, Apo A-I, and NEFA. Different leptin levels by sex may contribute to explain the sex-dependent association of the SNPs with these variables.
脂肪甘油三酯脂酶（ perilipin (）基因的变异与肥胖及其相关改变有关，但不同的营养状态似乎对这些关联的差异有影响。在我们的研究中，我们研究了 基因座的几个多态性与肥胖和儿童脂质谱的关系，然后分析了血浆瘦素水平对这些关联的中介作用。通过 RT-PCR 在 1264 名 6-8 岁的儿童中分析了脂肪甘油三酯脂酶（ perilipin (）基因中的单核苷酸多态性（SNPs）rs894160、rs1052700 和 rs2304795，以及 基因中的 rs35568725。我们的结果显示，rs1052700 与载脂蛋白（Apo）A-I 水平在男孩和女孩中的关联相反，TT 基因型携带者在男孩中显示出显著更高的 Apo A-I 水平，而在女孩中显示出显著更低的 Apo A-I 水平。SNP rs35568725 与高密度脂蛋白胆固醇（HDL-cholesterol）、Apo A-I 和非酯化脂肪酸（NEFA）的显著关联仅在男孩中观察到，而在女孩中未观察到。在男孩和女孩中，所研究的 SNP 与体重指数（BMI）、NEFA 和 Apo A-I 的关联因瘦素浓度而异。我们描述了血浆瘦素水平在 基因和 基因中 SNPs 与 BMI、Apo A-I 和 NEFA 之间的关联中的中介作用。不同性别之间的瘦素水平可能有助于解释 SNPs 与这些变量之间的性别依赖性关联。
