Smith Caren E, Tucker Katherine L, Yiannakouris Nikos, Garcia-Bailo Bibiana, Mattei Josiemer, Lai Chao-Qiang, Parnell Laurence D, Ordovás José M
Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University School of Medicine, Boston, MA 02111, USA.
J Nutr. 2008 Oct;138(10):1852-8. doi: 10.1093/jn/138.10.1852.
Perilipin (PLIN) is the major protein surrounding lipid droplets in adipocytes and regulates adipocyte metabolism by modulating the interaction between lipases and triacylglycerol stores. Associations between PLIN gene polymorphisms and obesity risk have been described, but interactions with dietary macronutrients require further attention. We examined whether dietary macronutrients (e.g. carbohydrates and fats) modulated the associations of the common PLIN 11482G > A (rs894160) single nucleotide polymorphism with obesity. We studied a population-based sample of Caribbean-origin Hispanics (n = 920, aged 45-74 y) living in the Boston area. Obesity measures (waist and hip circumference, BMI) did not differ between GG subjects and carriers of the A allele (GA and AA). In multivariate linear regression models, we found a significant interaction between complex carbohydrate intake as a continuous variable and PLIN 11482 G > A genotype for waist circumference (P = 0.002). By dichotomizing complex carbohydrate intake, we found significantly different effects across PLIN 11482G > A genotypes. When complex carbohydrate intake was <144 g/d, waist circumference was larger in PLIN 11482G > A carriers (P = 0.024). Conversely, when complex carbohydrate intake was >/=144 g/d, waist and hip circumferences were less in PLIN 11482G > A carriers (P < 0.05). These interactions were not found for simple sugars or total carbohydrates. We identified a significant gene-diet interaction associated with obesity at the PLIN locus. In subjects with higher complex carbohydrate intake, the minor allele was protective against obesity, whereas in subjects with lower carbohydrate intake, the minor allele was associated with increased obesity. These interactions may be relevant to dietary management of obesity.
perilipin（PLIN）是脂肪细胞中围绕脂滴的主要蛋白质，通过调节脂肪酶与三酰甘油储存之间的相互作用来调节脂肪细胞代谢。已有研究描述了PLIN基因多态性与肥胖风险之间的关联，但与膳食常量营养素的相互作用仍需进一步关注。我们研究了膳食常量营养素（如碳水化合物和脂肪）是否会调节常见的PLIN 11482G>A（rs894160）单核苷酸多态性与肥胖之间的关联。我们对居住在波士顿地区的加勒比裔西班牙裔人群样本（n = 920，年龄45 - 74岁）进行了研究。GG受试者与A等位基因携带者（GA和AA）之间的肥胖测量指标（腰围、臀围、BMI）没有差异。在多变量线性回归模型中，我们发现作为连续变量的复合碳水化合物摄入量与PLIN 11482 G>A基因型在腰围方面存在显著交互作用（P = 0.002）。通过将复合碳水化合物摄入量进行二分法分析，我们发现不同PLIN 11482G>A基因型的影响存在显著差异。当复合碳水化合物摄入量<144 g/d时，PLIN 11482G>A携带者的腰围更大（P = 0.024）。相反，当复合碳水化合物摄入量≥144 g/d时，PLIN 11482G>A携带者的腰围和臀围更小（P < 0.05）。对于单糖或总碳水化合物未发现这些交互作用。我们在PLIN基因座发现了与肥胖相关的显著基因 - 饮食交互作用。在复合碳水化合物摄入量较高的受试者中，次要等位基因对肥胖具有保护作用，而在碳水化合物摄入量较低的受试者中，次要等位基因与肥胖增加有关。这些交互作用可能与肥胖的饮食管理相关。
