肌醇焦磷酸对蛋白质的磷酸化作用。

Phosphorylation of proteins by inositol pyrophosphates.

作者信息

Saiardi Adolfo, Bhandari Rashna, Resnick Adam C, Snowman Adele M, Snyder Solomon H

机构信息

Department of Neuroscience, Johns Hopkins University, School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA.

出版信息

Science. 2004 Dec 17;306(5704):2101-5. doi: 10.1126/science.1103344.

DOI:10.1126/science.1103344

PMID:15604408

Abstract

The inositol pyrophosphates IP7 and IP8 contain highly energetic pyrophosphate bonds. Although implicated in various biologic functions, their molecular sites of action have not been clarified. Using radiolabeled IP7, we detected phosphorylation of multiple eukaryotic proteins. We also observed phosphorylation of endogenous proteins by endogenous IP7 in yeast. Phosphorylation by IP7 is nonenzymatic and may represent a novel intracellular signaling mechanism.

摘要

肌醇焦磷酸IP7和IP8含有高能焦磷酸键。尽管它们参与了多种生物学功能，但其分子作用位点尚未明确。我们使用放射性标记的IP7检测到多种真核生物蛋白质发生了磷酸化。我们还观察到酵母中内源性IP7对内源性蛋白质的磷酸化作用。IP7介导的磷酸化是非酶促的，可能代表一种新的细胞内信号传导机制。