Villadsen Dorthe, Smith Steven M
Institute of Cell and Molecular Biology, University of Edinburgh, The King's Buildings, Mayfield Road, Edinburgh, EH9 3JH, UK.
Plant Mol Biol. 2004 Jul;55(4):467-77. doi: 10.1007/s11103-004-1050-0.
The response of some plant genes to glucose analogues 3-O-methylglucose (3OMG) or 6-deoxyglucose (6DOG) has been cited as evidence for metabolism-independent glucose signalling. To analyse such signalling using a genetic approach, we sought to identify Arabidopsis glucose-responsive genes which also respond to 3OMG and 6DOG in seedlings. Microarray analysis of gene expression in glucose-treated seedlings and RT-PCR analysis of glucose-treated leaf sections identified more than 200 glucose-responsive genes, but none responded to 3OMG or 6DOG. These data together with other published data on individual genes fail to identify any Arabidopsis sugar-responsive genes which also respond to 3OMG or 6DOG.
一些植物基因对葡萄糖类似物3 - O - 甲基葡萄糖（3OMG）或6 - 脱氧葡萄糖（6DOG）的反应已被引为代谢非依赖性葡萄糖信号传导的证据。为了用遗传学方法分析这种信号传导，我们试图鉴定拟南芥中在幼苗期对葡萄糖有反应且也对3OMG和6DOG有反应的葡萄糖响应基因。对葡萄糖处理的幼苗进行基因表达微阵列分析以及对葡萄糖处理的叶片切片进行逆转录聚合酶链反应（RT-PCR）分析，鉴定出了200多个葡萄糖响应基因，但没有一个对3OMG或6DOG有反应。这些数据以及其他已发表的关于单个基因的数据都未能鉴定出任何对3OMG或6DOG也有反应的拟南芥糖响应基因。
