与其他方法相比，猪流行性腹泻病毒的免疫组织化学检测
Immunohistochemical detection of porcine epidemic diarrhea virus compared to other methods.
作者信息
Guscetti F, Bernasconi C, Tobler K, Van Reeth K, Pospischil A, Ackermann M
机构信息
Institute of Veterinary Pathology, University of Zurich, Switzerland.
出版信息
Clin Diagn Lab Immunol. 1998 May;5(3):412-4. doi: 10.1128/CDLI.5.3.412-414.1998.
Abstract
An immunohistochemistry method using formalin-fixed tissues, a direct immunofluorescence method using cryostat sections, an enzyme-linked immunosorbent assay (ELISA), and a PCR method were compared for diagnosis in a litter of weaned pigs that had been experimentally inoculated with wild-type porcine epidemic diarrhea virus (PEDV) and killed between 6 and 60 h after onset of diarrhea. The immunohistochemistry method proved to be as reliable as direct immunofluorescence for diagnosis of PEDV in tissues collected postmortem. The good reliability of ELISA for investigating clinical samples was confirmed, whereas the PCR method used was ineffective.
摘要
对一窝经实验接种野生型猪流行性腹泻病毒（PEDV）并在腹泻开始后6至60小时内扑杀的断奶仔猪，比较了使用福尔马林固定组织的免疫组织化学方法、使用冰冻切片的直接免疫荧光方法、酶联免疫吸附测定（ELISA）和PCR方法用于诊断的效果。结果表明，免疫组织化学方法在死后采集的组织中诊断PEDV时与直接免疫荧光法一样可靠。ELISA用于检测临床样本的可靠性得到了证实，而所使用的PCR方法无效。
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