Sharma A, Inagaki S, Sigurdson W, Kuramitsu H K
Department of Oral Biology, School of Dental Medicine, State University of New York, Buffalo, New York 14214, USA.
Oral Microbiol Immunol. 2005 Feb;20(1):39-42. doi: 10.1111/j.1399-302X.2004.00175.x.
During dental plaque formation, the interaction of different organisms is important in the development of complex communities. Fusobacterium nucleatum is considered a 'bridge-organism' that facilitates colonization of other bacteria by coaggregation-mediated mechanisms and possibly by making the environment conducive for oxygen intolerant anaerobes. These studies were carried out to determine whether coaggregation between F. nucleatum and Tannerella forsythia is important in the formation of mixed species biofilms. Further, the role of BspA protein, a surface adhesin of T. forsythia, in coaggregation and biofilm formation was investigated. The results showed the development of synergistic mixed biofilms of F. nucleatum and T. forsythia when these bacteria were cocultured. The BspA protein was not involved in biofilm formation. Though BspA plays a role in coaggregation with F. nucleatum, presumably other adhesins are also involved. The synergistic biofilm formation between the two species was dependent on cell-cell contact and soluble components of the bacteria were not required. This study demonstrates that there is a positive synergy between F. nucleatum and T. forsythia in the development of mixed biofilms and that the cell-cell interaction is essential for this phenomenon.
在牙菌斑形成过程中，不同微生物之间的相互作用对于复杂群落的发展至关重要。具核梭杆菌被认为是一种“桥梁微生物”，它通过共聚集介导的机制，可能还通过营造有利于耐氧厌氧菌生存的环境，促进其他细菌的定殖。开展这些研究是为了确定具核梭杆菌与福赛斯坦纳菌之间的共聚集在混合物种生物膜形成过程中是否重要。此外，还研究了福赛斯坦纳菌的表面黏附素BspA蛋白在共聚集和生物膜形成中的作用。结果表明，当这两种细菌共培养时，会形成具核梭杆菌与福赛斯坦纳菌的协同混合生物膜。BspA蛋白不参与生物膜形成。虽然BspA在与具核梭杆菌的共聚集中发挥作用，但推测其他黏附素也参与其中。这两个物种之间的协同生物膜形成依赖于细胞间接触，不需要细菌的可溶性成分。这项研究表明，具核梭杆菌与福赛斯坦纳菌在混合生物膜形成过程中存在积极的协同作用，且细胞间相互作用对于这一现象至关重要。
