Zhang Yong-xin, Zhang Ying, Zhang Qi-wei
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2003 Oct;28(10):951-3.
To examine the factors in extracting the minute powder of processed rhizome of Corydalis with vinegar for its application.
The one-way variation analysis was used for experimental design. Tetrahydropalmatine was determined with HPLC method. The samples were separated on a Diamonsil C18 column with mobile phase of acetonitrile-TEA phosphate buffer (pH 7.0) (50:50) and detection wavelength was 280 mm.
The extraction conditions were established as follows: extraction temperature is 60 degrees C, water-added amount is 20 times the weight of the powder, and extraction time is 30 minutes.
The extraction rate of the tetrahydropalmatine in the minute power is about 10% higher than that of the processed rhizome with vinegar. But less water amount, less time, and lower temperature were needed for extraction. The application of minute powder can reduce energy consumption and raise economic benefit in industrial production.
考察醋制延胡索细粉的提取因素及其应用。
采用单因素方差分析进行实验设计。用高效液相色谱法测定延胡索乙素。样品在Diamonsil C18柱上分离，流动相为乙腈-磷酸三乙胺缓冲液（pH 7.0）（50:50），检测波长为280nm。
确定提取条件如下：提取温度为60℃，加水量为粉末重量的20倍，提取时间为30分钟。
细粉中延胡索乙素的提取率比醋制延胡索高约10%。但提取所需水量少、时间短、温度低。细粉的应用可降低工业生产中的能耗并提高经济效益。
