Okada M, Klimpel G R, Kuppers R C, Henney C S
J Immunol. 1979 Jun;122(6):2527-33.
Within 15 hr of establishment of a murine mixed lymphocyte culture, a soluble mediator was produced that was capable of augmenting primary cytotoxic responses to alloantigen. The factor did not induce responsiveness in the absence of antigen, since the amplified response seen in its presence was specific for the stimulating alloantigen. The factor did not therefore appear to function by polyclonal activation of cytotoxic precursor cells. Production of the amplifying factor(s) was induced by unfractionated spleen cells, but not by cells subjected to UV irradiation or to sonication, making it likely that this deficiency is the basis of the well-documented failure of these stimulator cells to induce primary cytotoxic responses. The amplifying effects of the factor were distinctive from, but synergistic with, those of 2-mercaptoethanol. Production of the amplifying mediator did not require cell division but was dependent upon the presence of Lyt 1 + cells. On the other hand, Lyt 2 + cells were not needed for mediator production, but served as target cell population on which the factor exerted its action. These findings are compatible with the hypothesis that direct T-T cell collaboration can amplify the differentiation of cytotoxic cells.
在建立小鼠混合淋巴细胞培养后的15小时内，产生了一种可溶性介质，它能够增强对同种异体抗原的初次细胞毒性反应。该因子在无抗原时不会诱导反应性，因为在其存在下观察到的放大反应对刺激的同种异体抗原具有特异性。因此，该因子似乎不是通过细胞毒性前体细胞的多克隆激活发挥作用。未分级的脾细胞可诱导产生放大因子，但经紫外线照射或超声处理的细胞则不能，这表明这种缺陷可能是这些刺激细胞未能诱导初次细胞毒性反应的原因，这一点已有充分记录。该因子的放大作用与2-巯基乙醇的作用不同，但具有协同性。产生放大介质不需要细胞分裂，但依赖于Lyt 1 +细胞的存在。另一方面，产生介质不需要Lyt 2 +细胞，但Lyt 2 +细胞作为该因子发挥作用的靶细胞群体。这些发现与直接T - T细胞协作可放大细胞毒性细胞分化的假说相符。
