Zhuang Y H, Bläuer M, Pekki A, Tuohimaa P
Department of Biomedical Sciences, University of Tampere, Finland.
J Steroid Biochem Mol Biol. 1992 Mar;41(3-8):693-6. doi: 10.1016/0960-0760(92)90406-9.
Location of the androgen receptor (AR) before and after dihydrotestosterone (DHT) administration was studied in 6 castrated and 2 normal male rats, as well as in MG-63 human osteosarcoma cell culture. Two days after castration, rats were injected with DHT and sacrificed 0, 6 and 24 h later. Cryosections of ventral prostate and seminal vesicle were stained with a polyclonal anti-AR antibody. Cultured MG-63 cells were also stained similarly. The intensity of immunoreaction was measured semiquantitatively by computer-assisted image analysis. In both normal and castrated rats, a positive reaction was seen mainly in the nuclei of epithelial cells and stromal cells of the prostate and seminal vesicle, as well as in those of smooth muscle cells of the seminal vesicle. AR immunoreactivity was up-regulated by DHT, it decreased clearly in both organs after castration. Nuclear AR and its up-regulation by androgen were also seen in MG-63 cells. At the immunoelectron microscopy, silver enhanced gold particles were predominantly found in the heterochromatin of cell nuclei. Treatment with DHT caused a decondensation of the heterochromatin and AR was more dispersed. Thus, AR appears to be nuclear independently of the ligand.
在6只去势雄鼠、2只正常雄鼠以及MG-63人骨肉瘤细胞培养物中，研究了给予二氢睾酮（DHT）前后雄激素受体（AR）的定位。去势两天后，给大鼠注射DHT，并在0、6和24小时后处死。用多克隆抗AR抗体对腹侧前列腺和精囊的冰冻切片进行染色。培养的MG-63细胞也进行类似染色。通过计算机辅助图像分析对免疫反应强度进行半定量测量。在正常和去势大鼠中，阳性反应主要见于前列腺和精囊的上皮细胞、基质细胞以及精囊平滑肌细胞的细胞核中。AR免疫反应性被DHT上调，去势后两个器官中的反应性均明显降低。在MG-63细胞中也可见到核AR及其被雄激素上调的现象。在免疫电子显微镜下，银增强金颗粒主要见于细胞核的异染色质中。DHT处理导致异染色质解聚，AR分布更分散。因此，AR似乎独立于配体而存在于细胞核中。
