Kumar V L, Majumder P K, Kumar V
Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
Mol Cell Biochem. 1997 Jun;171(1-2):133-8. doi: 10.1023/a:1006805503284.
We have studied the effect of androgenic deprivation on the level of androgen receptor transcript in the rat ventral prostate. The rats were treated with estradiol benzoate, flutamide and [D Trp6, des Gly10]gonadotropin releasing hormone (GnRH) for different time periods. These treatments produced a significant decrease in the weight of prostate. Total RNA isolated from the ventral prostates was hybridized with the cDNA probe for androgen receptor. Densitometric analysis of the autoradiographic signal revealed a rise in the level of androgen receptor RNA following treatment of rats with estradiol benzoate and flutamide. Treatment of rats with [D Trp6, des Gly10] GnRH brought about a transient rise in the level of androgen receptor RNA. Thus, our results indicate that androgenic deprivation up-regulates the level of androgen receptor transcript.
我们研究了雄激素剥夺对大鼠腹侧前列腺中雄激素受体转录水平的影响。用苯甲酸雌二醇、氟他胺和[D-Trp6，des Gly10]促性腺激素释放激素（GnRH）对大鼠进行不同时间段的处理。这些处理使前列腺重量显著减轻。从腹侧前列腺分离的总RNA与雄激素受体的cDNA探针杂交。对放射自显影信号的光密度分析显示，用苯甲酸雌二醇和氟他胺处理大鼠后，雄激素受体RNA水平升高。用[D-Trp6，des Gly10]GnRH处理大鼠导致雄激素受体RNA水平短暂升高。因此，我们的结果表明，雄激素剥夺上调了雄激素受体转录物的水平。
