Lee Eung-Je, Lee Su-Hee, Kim Hae-Won, Kong Young-Min, Kim Hyoun-Ee
School of Materials Science and Engineering, Seoul National University, Seoul 151-744, Korea.
Biomaterials. 2005 Jun;26(18):3843-51. doi: 10.1016/j.biomaterials.2004.10.019.
In this report, a series of fluoridated apatite coatings were obtained by the electron-beam deposition method. The fluoridation of the apatite was aimed to improve the stability of the coating and elicit the fluorine effect, which is useful in the dental restoration area. Apatites fluoridated at different levels were used as initial evaporants for the coatings. The as-deposited coatings were amorphous, but after heat treatment at 500 degrees C for 1 h, the coatings crystallized well to an apatite phase without forming any cracks. The adhesion strengths of the as-deposited coatings were about 40 MPa. After heat treatment at 500 degrees C, the strengths of the pure HA and FA coatings decreased to about 20 MPa, however, the partially fluoridated coatings maintained their initial strength. The dissolution rate of the fluoridated coatings was lower than that of the pure HA coating, and the rate was the lowest in the coatings with 25% and 50% fluorine substitutions. The osteoblast-like cells responded to the coatings in a similar manner to the dissolution behavior. The cells on the fluoridated coatings showed a lower (p < 0.05) proliferation level compared to those on the pure HA coating. The alkaline phosphatase activity of the cells was slightly lower than that on the pure HA coating, but this difference was not statistically significant.
在本报告中，通过电子束沉积法获得了一系列含氟磷灰石涂层。对磷灰石进行氟化旨在提高涂层的稳定性并引发氟效应，这在牙齿修复领域是有用的。不同氟化程度的磷灰石用作涂层的初始蒸发源。沉积后的涂层是非晶态的，但在500℃下热处理1小时后，涂层结晶良好，形成磷灰石相且未形成任何裂纹。沉积后涂层的附着强度约为40MPa。在500℃热处理后，纯HA和FA涂层的强度降至约20MPa，然而，部分氟化涂层保持了其初始强度。氟化涂层的溶解速率低于纯HA涂层，且在氟取代率为25%和50%的涂层中速率最低。成骨样细胞对涂层的反应与溶解行为相似。与纯HA涂层上的细胞相比，氟化涂层上的细胞增殖水平较低（p<0.05）。细胞的碱性磷酸酶活性略低于纯HA涂层上的细胞，但这种差异无统计学意义。
