Kim Hae-Won, Kim Hyoun-Ee, Knowles Jonathan C
School of Materials Science and Engineering, Seoul National University, Seoul 151-742, South Korea.
Biomaterials. 2004 Aug;25(17):3351-8. doi: 10.1016/j.biomaterials.2003.09.104.
Hydroxyapatite (HA) and fluor-hydroxyapatite (FHA) films were deposited on a titanium substrate using a sol-gel technique. Different concentrations of F- were incorporated into the apatite structure during the sol preparation. Typical apatite structures were obtained for all coatings after dipping and subsequent heat treatment at 500 degrees C. The films obtained were uniform and dense, with a thickness of approximately 5 microm. The dissolution rate of the coating layer decreased with increasing F- incorporation within the apatite structure, which demonstrates the possibility of tailoring the solubility by a functional gradient coating of HA and FHA. The cell proliferation rate on the coating layer decreased slightly with increasing F- incorporation. The alkaline phosphatase (ALP) activity of the cells on all the HA and FHA coated samples showed much higher expression levels compared to pure Ti. This confirmed the improved activity of cell functions on the substrates with the sol-gel coating treatment.
采用溶胶-凝胶技术在钛基底上沉积羟基磷灰石（HA）和氟羟基磷灰石（FHA）薄膜。在溶胶制备过程中，将不同浓度的F-掺入磷灰石结构中。浸渍后并在500℃进行后续热处理，所有涂层均获得了典型的磷灰石结构。所获得的薄膜均匀且致密，厚度约为5微米。随着磷灰石结构中F-掺入量的增加，涂层的溶解速率降低，这表明通过HA和FHA的功能梯度涂层来调整溶解度是可能的。随着F-掺入量的增加，涂层上的细胞增殖速率略有下降。与纯钛相比，所有HA和FHA涂层样品上细胞的碱性磷酸酶（ALP）活性均表现出更高的表达水平。这证实了溶胶-凝胶涂层处理后基底上细胞功能活性的提高。
