Dowd Patrick F, Barnett C Jason, Johnson Eric T, Beck James J
U.S.D.A., Agricultural Research Service, National Center for Agricultural Utilization Research, Crop Bioprotection Research Unit, 1815 N. University St., Peoria, IL 61614, USA.
Mycopathologia. 2004 Nov;158(4):431-40. doi: 10.1007/s11046-004-3862-5.
PCR analysis was used to detect Fusarium species generically, as well as the mycotoxin-producing species F. subglutinans, F. proliferatum, and F. verticillioides in leaf axil and other maize tissues during ear fill in a multiyear study in central Illinois. The frequency of Fusarium detected varied from site to site and year to year. Fusarium was generically detected more frequently in leaf axil material than in leaf/husk lesions. In two growing seasons, the leaf axil samples were also tested for the presence of the mycotoxin producing species F. proliferatum, F. subglutinans, and F. verticillioides. Overall, F. proliferatum and F. verticillioides were detected less often than F. subglutinans. Fusarium was generically and specifically detected most commonly where visible fungal growth was present in leaf axil material.
在伊利诺伊州中部进行的一项多年研究中，采用聚合酶链反应（PCR）分析从总体上检测镰刀菌属物种，以及在玉米灌浆期叶腋和其他玉米组织中产生霉菌毒素的亚粘团镰刀菌、轮枝镰刀菌和层出镰刀菌。检测到的镰刀菌频率因地点和年份而异。从总体上看，在叶腋材料中检测到镰刀菌的频率高于叶/苞叶病斑。在两个生长季节中，还对叶腋样本进行了检测，以确定是否存在产生霉菌毒素的层出镰刀菌、亚粘团镰刀菌和轮枝镰刀菌。总体而言，检测到层出镰刀菌和轮枝镰刀菌的频率低于亚粘团镰刀菌。在叶腋材料中出现可见真菌生长的地方，最常从总体上和特异性地检测到镰刀菌。
