Liu Xiao-Hong, Lu Jian-Ping, Zhang Lei, Dong Bo, Min Hang, Lin Fu-Cheng
Biotechnology Institute, Zhejiang University, Kaixuan Road 268, Hangzhou 310029, China.
Eukaryot Cell. 2007 Jun;6(6):997-1005. doi: 10.1128/EC.00011-07. Epub 2007 Apr 6.
We isolated an MgATG1 gene encoding a serine/threonine protein kinase from the rice blast fungus Magnaporthe grisea. In the DeltaMgatg1 mutant, in which the MgATG1 gene had been deleted, autophagy was blocked; the mutant also showed fewer lipid droplets in its conidia, lower turgor pressure of the appressorium, and such defects in morphogenesis as delayed initiation and slower germination of conidia. As a result of lower turgor pressure of the appressorium, the DeltaMgatg1 mutant lost its ability to penetrate and infect the two host plants, namely, rice and barley. However, normal values of the parameters and infective abilities were restored on reintroducing an intact copy of the MgATG1 gene into the mutant. Autophagy is thus necessary for turnover of organic matter during the formation of conidia and appressoria and for normal development and pathogenicity in M. grisea.
我们从稻瘟病菌（Magnaporthe grisea）中分离出了一个编码丝氨酸/苏氨酸蛋白激酶的MgATG1基因。在MgATG1基因已被敲除的ΔMgatg1突变体中，自噬被阻断；该突变体的分生孢子中脂滴也较少，附着胞的膨压较低，并且在形态发生方面存在缺陷，如分生孢子的起始延迟和萌发较慢。由于附着胞的膨压较低，ΔMgatg1突变体失去了穿透和感染两种寄主植物（即水稻和大麦）的能力。然而，将完整的MgATG1基因拷贝重新导入突变体后，各项参数的正常值和感染能力得以恢复。因此，自噬对于稻瘟病菌分生孢子和附着胞形成过程中的有机物周转以及正常发育和致病性是必需的。
