Lu Qin, Lu Jian-Ping, Li Xiao-Dong, Liu Xiao-Hong, Min Hang, Lin Fu-Cheng
College of Life Sciences, Zhejiang University, Hangzhou, China.
J Zhejiang Univ Sci B. 2008 Jul;9(7):511-9. doi: 10.1631/jzus.B0820015.
In this study the MTP1 gene, encoding a type III integral transmembrane protein, was isolated from the rice blast fungus Magnaporthe oryzae. The Mtp1 protein is 520 amino acids long and is comparable to the Ytp1 protein of Saccharomyces cerevisiae with 46% sequence similarity. Prediction programs and MTP1-GFP (green fluorescent protein) fusion expression results indicate that Mtp1 is a protein located at several membranes in the cytoplasm. The functions of the MTP1 gene in the growth and development of the fungus were studied using an MTP1 gene knockout mutant. The MTP1 gene was primarily expressed at the hyphal and conidial stages and is necessary for conidiation and conidial germination, but is not required for pathogenicity. The Deltamtp1 mutant grew more efficiently than the wild type strain on non-fermentable carbon sources, implying that the MTP1 gene has a unique role in respiratory growth and carbon source use.
在本研究中，从稻瘟病菌稻瘟菌中分离出编码III型整合膜蛋白的MTP1基因。Mtp1蛋白长度为520个氨基酸，与酿酒酵母的Ytp1蛋白具有46%的序列相似性。预测程序和MTP1-绿色荧光蛋白（GFP）融合表达结果表明，Mtp1是一种位于细胞质中多个膜上的蛋白质。使用MTP1基因敲除突变体研究了MTP1基因在真菌生长和发育中的功能。MTP1基因主要在菌丝和分生孢子阶段表达，是分生孢子形成和分生孢子萌发所必需的，但致病性不需要该基因。Deltamtp1突变体在非发酵碳源上比野生型菌株生长得更有效，这意味着MTP1基因在呼吸生长和碳源利用中具有独特的作用。
