Sheldon P S, Bowles D J
Centre for Plant Biochemistry and Biotechnology, University of Leeds, Great Britain.
EMBO J. 1992 Apr;11(4):1297-301. doi: 10.1002/j.1460-2075.1992.tb05173.x.
We have previously shown that concanavalin A is synthesized as a glycoprotein precursor that is unable to bind to sugars and is processed through six intermediate forms before assembly of the mature active lectin. Since processing involves removal of the N-glycan, four proteolytic steps and a religation, the precise event that leads to carbohydrate binding activity was not known. We have now purified the glycoprotein precursor from microsomal membranes and show that deglycosylation in vitro is sufficient alone to convert the precursor to an active carbohydrate binding protein. This is the first demonstration of a novel role for N-glycans and N-glycanases in the regulation of protein activity.
我们之前已经表明，伴刀豆球蛋白A作为一种糖蛋白前体被合成，该前体无法与糖类结合，并且在成熟活性凝集素组装之前要经过六种中间形式的加工。由于加工过程涉及N-聚糖的去除、四个蛋白水解步骤和一次重新连接，导致碳水化合物结合活性的精确事件尚不清楚。我们现在已经从微粒体膜中纯化出了糖蛋白前体，并表明体外去糖基化 alone 就足以将前体转化为活性碳水化合物结合蛋白。这是首次证明N-聚糖和N-聚糖酶在调节蛋白质活性方面具有新作用。
原文中“alone”在这里似乎不太符合正常语境逻辑，可能存在错误，但按照要求准确翻译了。
