体外伴刀豆球蛋白A加工过程中的翻译后肽键形成
Post-translational peptide bond formation during concanavalin A processing in vitro.
作者信息
Sheldon P S, Keen J N, Bowles D J
机构信息
Centre for Plant Biochemistry, University of Leeds, U.K.
出版信息
Biochem J. 1996 Dec 15;320 ( Pt 3)(Pt 3):865-70. doi: 10.1042/bj3200865.
Abstract
Post-translational processing of concanavalin A (Con A) is complex, involving deglycosylation, proteolytic cleavage on the carboxy group side of asparagine residues and formation of a peptide bond de novo. This has been studied with the 125I-labelled Con A glycoprotein precursor as a substrate for processing in vitro. Extracts of immature jackbean cotyledons and the commercially available purified preparation of asparaginylendo-peptidase were able to catalyse the above processes. The processing resulted in the conversion of the 33.5 kDa inactive glycoprotein precursor into an active lectin. Processing activity was maximal at approx. pH 5.5. Evidence to support processing at authentic sites was obtained by observation of the release of 125I at positions in the sequence where tyrosine residues were present.
摘要
伴刀豆球蛋白A（Con A）的翻译后加工过程很复杂，包括去糖基化、天冬酰胺残基羧基侧的蛋白水解切割以及新肽键的形成。这一过程已使用125I标记的Con A糖蛋白前体作为体外加工的底物进行了研究。未成熟刀豆子叶提取物和市售的纯化天冬酰胺内肽酶制剂能够催化上述过程。加工过程导致33.5 kDa的无活性糖蛋白前体转化为活性凝集素。加工活性在约pH 5.5时最大。通过观察序列中酪氨酸残基所在位置的125I释放，获得了支持在真实位点进行加工的证据。
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本文引用的文献
1
Peptide splicing in the vacuolar ATPase subunit A from Candida tropicalis.热带假丝酵母液泡ATP酶亚基A中的肽剪接
J Biol Chem. 1993 Apr 5;268(10):7372-81.
2
3
Molecular characterization of a vacuolar processing enzyme related to a putative cysteine proteinase of Schistosoma mansoni.与曼氏血吸虫假定半胱氨酸蛋白酶相关的液泡加工酶的分子特征分析
Plant Cell. 1993 Nov;5(11):1651-9. doi: 10.1105/tpc.5.11.1651.
4
Posttranslational processing of a new class of hydroxyproline-containing proteins. Prolyl hydroxylation and C-terminal cleavage of tobacco (Nicotiana tabacum) vacuolar chitinase.一类含羟脯氨酸新蛋白的翻译后加工。烟草（Nicotiana tabacum）液泡几丁质酶的脯氨酰羟化和C末端切割。
Plant Physiol. 1993 Apr;101(4):1239-47. doi: 10.1104/pp.101.4.1239.
5
In vitro protein splicing of purified precursor and the identification of a branched intermediate.纯化前体的体外蛋白质剪接及分支中间体的鉴定。
Cell. 1993 Dec 31;75(7):1371-7. doi: 10.1016/0092-8674(93)90623-x.
6
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7
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Biochim Biophys Acta. 1981 Feb 13;657(2):457-67. doi: 10.1016/0005-2744(81)90331-4.
8
Amino acid sequence and variant forms of favin, a lectin from Vicia faba.蚕豆凝集素favin的氨基酸序列及变异形式
J Biol Chem. 1982 Apr 25;257(8):4473-83.
9
The biosynthesis and primary structure of pea seed lectin.豌豆种子凝集素的生物合成与一级结构
J Biol Chem. 1983 Aug 10;258(15):9544-9.
10
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