Imanishi Nobuko, Mantani Naoki, Sakai Shinya, Sato Miyuki, Katada Yuko, Ueda Kyouka, Terasawa Katsutoshi, Ochiai Hiroshi
Department of Oriental Medicine, Toyama Medical and Pharmaceutical University, Sugitani 2630, Toyama 930-0194, Japan.
Am J Chin Med. 2004;32(5):727-35. doi: 10.1142/S0192415X04002302.
We have investigated the effect of Zingiber offifinale Rosc. (ZOR) on macrophage-inducible nitric oxide (NO) synthase (macNOS) mRNA expression and NO production in RAW264.7 cells, a murine macrophage cell line; 100 microg/ml ZOR can induce macNOS mRNA expression, but induction effects at a dose below 10 microg/ml were weak or negligible. Kinetic studies showed that macNOS mRNA can be detected from 4 hours to 24 hours after dosing, with a peak at 8 hours. In accordance with the induction of macNOS mRNA expression, NO concentrations increased from 3.4 microM at 2 hours to almost 150 microM at 24 hours, reflecting a longer period of macNOS mRNA expression. The activity of ZOR can be considered to contribute, at least in part, to the beneficial effects of ZOR through the macNOS-mediated activation of the biodefense mechanism.
我们研究了姜（Zingiber offifinale Rosc.，ZOR）对小鼠巨噬细胞系RAW264.7细胞中巨噬细胞诱导型一氧化氮（NO）合酶（macNOS）mRNA表达及NO生成的影响；100μg/ml的ZOR可诱导macNOS mRNA表达，但剂量低于10μg/ml时诱导作用微弱或可忽略不计。动力学研究表明，给药后4小时至24小时可检测到macNOS mRNA，8小时达到峰值。与macNOS mRNA表达的诱导情况一致，NO浓度从2小时时的3.4μM增加至24小时时的近150μM，这反映出macNOS mRNA表达持续时间更长。ZOR的活性至少部分有助于通过macNOS介导的生物防御机制激活而产生ZOR的有益作用。
