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快速分离质粒DNA的方法及其在流行病学分析中的应用

Rapid procedure for isolation of plasmid DNA and application to epidemiological analysis.

作者信息

Takahashi S, Nagano Y

出版信息

J Clin Microbiol. 1984 Oct;20(4):608-13. doi: 10.1128/jcm.20.4.608-613.1984.

DOI:10.1128/jcm.20.4.608-613.1984
PMID:6092432
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC271394/
Abstract

A rapid and simple plasmid isolation procedure was developed for the epidemiological analysis of plasmid-mediated antimicrobial resistance. By this method, plasmid DNAs ranging in molecular weight between 2.0 and 122 X 10(6) could be detected. Various bacteria, such as strains of the family Enterobacteriaceae, Pseudomonas aeruginosa, Haemophilus influenzae, and Staphylococcus aureus, could be analyzed. The plasmid DNA obtained could be directly used for restriction endonuclease analysis without further purification. In addition, this method made it possible to analyze several cultures at the same time.

摘要

开发了一种快速简便的质粒分离程序，用于质粒介导的抗菌药物耐药性的流行病学分析。通过这种方法，可以检测分子量在2.0至122×10⁶之间的质粒DNA。可以分析各种细菌，如肠杆菌科菌株、铜绿假单胞菌、流感嗜血杆菌和金黄色葡萄球菌。获得的质粒DNA无需进一步纯化即可直接用于限制性内切酶分析。此外，这种方法使得同时分析几种培养物成为可能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da5/271394/b5c96c43649c/jcm00123-0042-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da5/271394/d1bf6fd67067/jcm00123-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da5/271394/a22718dafd64/jcm00123-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da5/271394/83bea8f711f0/jcm00123-0041-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da5/271394/d0bc7dddcc84/jcm00123-0042-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da5/271394/b5c96c43649c/jcm00123-0042-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da5/271394/d1bf6fd67067/jcm00123-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da5/271394/a22718dafd64/jcm00123-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da5/271394/83bea8f711f0/jcm00123-0041-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da5/271394/d0bc7dddcc84/jcm00123-0042-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0da5/271394/b5c96c43649c/jcm00123-0042-b.jpg

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Characterization of chloramphenicol-resistant Haemophilus influenzae.耐氯霉素流感嗜血杆菌的特性分析
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Isolation and characterization of a Pseudomonas aeruginosa from a virgin Brazilian Amazon region with potential to degrade atrazine.从巴西亚马逊地区原始区域分离出一株具有潜在降解莠去津能力的铜绿假单胞菌及其特性研究。
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Characterization of multi-antibiotic-resistant Escherichia coli Isolated from beef cattle in Japan.从日本肉牛中分离出的多重耐药性大肠杆菌的特性分析
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