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十六烷基氯化吡啶鎓可用于从长期保存的痰液中分离结核分枝杆菌。

Cetyl-pyridinium chloride is useful for isolation of Mycobacterium tuberculosis from sputa subjected to long-term storage.

作者信息

Pardini Manuela, Varaine Francis, Iona Elisabetta, Arzumanian Erchanik, Checchi Francesco, Oggioni Marco Rinaldo, Orefici Graziella, Fattorini Lanfranco

机构信息

Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.

出版信息

J Clin Microbiol. 2005 Jan;43(1):442-4. doi: 10.1128/JCM.43.1.442-444.2005.

DOI:10.1128/JCM.43.1.442-444.2005
PMID:15635010
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC540128/
Abstract

Recovery of Mycobacterium tuberculosis from sputa treated with cetyl-pyridinium chloride (CPC) and stored for 20 +/- 9 days was significantly higher than that from sputa that were untreated and processed by the N-acetyl-L-cisteine-NaOH method. Addition of CPC is useful for isolation of M. tuberculosis from sputa subjected to long-term storage received from remote areas of the world.

摘要

用十六烷基氯化吡啶（CPC）处理并储存20±9天的痰液中结核分枝杆菌的回收率显著高于未处理且采用N-乙酰-L-半胱氨酸-氢氧化钠方法处理的痰液。添加CPC有助于从世界偏远地区送来的长期储存痰液中分离结核分枝杆菌。

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本文引用的文献

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Comparison of sodium carbonate, cetyl-pyridinium chloride, and sodium borate for preservation of sputa for culture of Mycobacterium tuberculosis.碳酸钠、十六烷基氯化吡啶和硼酸钠用于保存痰液以培养结核分枝杆菌的比较。
J Clin Microbiol. 2003 Sep;41(9):4487-8. doi: 10.1128/JCM.41.9.4487-4488.2003.
2
Bacteriological follow-up of tuberculosis treatment: a comparative study of smear microscopy and culture results at the second month of treatment.结核病治疗的细菌学随访：治疗第二个月涂片显微镜检查与培养结果的比较研究
Int J Tuberc Lung Dis. 2002 Oct;6(10):909-12.
3
Isolation of tubercle bacilli from sputum samples of patients in the field studies by the cetylpyridinium chloride-sodium chloride & sodium hydroxide methods.通过十六烷基吡啶氯化物 - 氯化钠及氢氧化钠法从现场研究中患者的痰液样本中分离结核杆菌。
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Use of cetylpyridinium chloride and sodium chloride for the decontamination of sputum specimens that are transported to the laboratory for the isolation of Mycobacterium tuberculosis.使用十六烷基氯化吡啶和氯化钠对运送至实验室用于分离结核分枝杆菌的痰液标本进行净化处理。
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Effect of cetylpyridinium chloride on pathogenic fungi and Nocardia asteroides in sputum.十六烷基氯化吡啶对痰液中致病真菌和星形诺卡菌的作用。
J Clin Microbiol. 1976 Mar;3(3):272-6. doi: 10.1128/jcm.3.3.272-276.1976.
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