评估从拭子中分离溃疡分枝杆菌的去污方案。
Evaluating decontamination protocols for the isolation of Mycobacterium ulcerans from swabs.
作者信息
Owusu Enid, Newman Mercy J, Akumwena Amos, Bannerman Elizabeth, Pluschke Gerd
机构信息
Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana.
Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana.
出版信息
BMC Microbiol. 2017 Jan 5;17(1):2. doi: 10.1186/s12866-016-0918-x.
BACKGROUND
Mycobacterium ulcerans (M. ulcerans) is the causative agent of Buruli Ulcer (BU) disease. In order to inhibit the growth of the microbial contaminants during culture of M. ulcerans, it is necessary to decontaminate BU samples with effective chemical agents. This study aimed at investigating some selected chemicals as potential decontamination agents for the isolation of M. ulcerans from swabs.
RESULTS
Povidone iodine at 0.5 and 1% exhibited the lowest contamination and recovery rate for microbial contaminants and M. ulcerans. The most effective decontamination method was the protocol using 2% cetylpyridinium chloride/4% sodium chloride (recovery rate = 53%, contamination rate = 14%). The observed difference between the recovery rate of 2% CPC/4% NaC and the other protocols was however not statistically significant (p = 0.76).
CONCLUSIONS
Two percent (2%) cetylpyridinium chloride/4% sodium chloride can be conveniently used as an alternative decontamination method for the isolation of M. ulcerans from swabs.
背景
溃疡分枝杆菌是布氏杆菌病的病原体。为了在溃疡分枝杆菌培养过程中抑制微生物污染物的生长，有必要用有效的化学试剂对布氏杆菌病样本进行去污处理。本研究旨在研究一些选定的化学物质作为从拭子中分离溃疡分枝杆菌的潜在去污剂。
结果
0.5%和1%的聚维酮碘对微生物污染物和溃疡分枝杆菌的污染和回收率最低。最有效的去污方法是使用2%十六烷基吡啶氯化物/4%氯化钠的方案（回收率=53%，污染率=14%）。然而，2% CPC/4% NaC的回收率与其他方案之间的差异无统计学意义（p = 0.76）。
结论
2%十六烷基吡啶氯化物/4%氯化钠可方便地用作从拭子中分离溃疡分枝杆菌的替代去污方法。
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