Smithwick R W, Stratigos C B, David H L
J Clin Microbiol. 1975 May;1(5):411-3. doi: 10.1128/jcm.1.5.411-413.1975.
A method is presented for the decontamination, liquefaction, and concentration of sputum specimens that are in transport more than 24 h. The method is inexpensive, and culture results compare well with those obtained with the accepted N-acetyl-L-cysteine and sodium hydroxide method for the isolation of tubercle bacilli. The working solution, 1% cetylpyridinium chloride and 2% sodium chloride, is mixed in equal volumes with sputum before the specimens are shipped. Tubercle bacilli remained viable after 8 days of exposure to this solution. Only Lowenstein-Jensen medium was used because the cetylpyridinium chloride in the inoculum remains active on 7H10 or other agar base media and partially inhibits the growth of tubercle bacilli.
本文介绍了一种用于对运输超过24小时的痰液标本进行去污、液化和浓缩的方法。该方法成本低廉，培养结果与用于分离结核杆菌的公认的N-乙酰-L-半胱氨酸和氢氧化钠方法所获得的结果相当。在标本运送前，将工作溶液（1%十六烷基吡啶氯化物和2%氯化钠）与痰液等体积混合。结核杆菌在接触该溶液8天后仍保持活力。仅使用了罗-琴培养基，因为接种物中的十六烷基吡啶氯化物在7H10或其他琼脂基础培养基上仍有活性，并部分抑制结核杆菌的生长。
