Saito Yoshihiro, Jinno Kiyokatsu, Greibrokk Tyge
School of Materials Science, Toyohashi University of Technology, Toyohashi 441-8580, Japan.
J Sep Sci. 2004 Dec;27(17-18):1379-90. doi: 10.1002/jssc.200401902.
Liquid chromatography on columns with small internal diameters has been reviewed as the intermediate technique between conventional liquid chromatography and microchip separations. The development of micro column separations in the early years has been described, starting with the papers of Horváth and co-workers and Ishii and co-workers, continuing into the first part of the eighties, then making a leap in time to recent innovations with small-bore columns. Based on internal diameters a classification of the different analytical HPLC columns has been suggested. The advantages of small-bore columns have been discussed, with particular emphasis on the advantage of coupling to concentration sensitive detectors when the sample amount is limited. Open tubular columns are treated as a part of the historic background. The recent developments include a brief look into the current status of monolithic columns, the use of packed nano columns and micro columns with electrospray mass spectrometry, and the potential of two-dimensional comprehensive liquid chromatography. Finally, the coupling of sample preparation to analytical columns and the future applications of the novel technological improvements to the microchip separation methods have been discussed.
内径较小的柱液相色谱已被视为传统液相色谱与微芯片分离之间的中间技术。本文回顾了早年微柱分离技术的发展历程，从霍瓦特及其同事、石井及其同事的论文开始，一直持续到八十年代上半叶，然后时间跳跃到近期小内径柱的创新成果。基于内径对不同的分析型高效液相色谱柱进行了分类。讨论了小内径柱的优点，特别强调了在样品量有限时与浓度敏感检测器联用的优势。开放管状柱作为历史背景的一部分进行了介绍。近期的发展包括简要介绍整体柱的现状、填充纳米柱和微柱与电喷雾质谱联用的应用，以及二维全二维液相色谱的潜力。最后，讨论了样品制备与分析柱的联用，以及新型技术改进在微芯片分离方法中的未来应用。
