马疱疹病毒4型糖蛋白G型特异性区域中另一个B细胞表位的鉴定。
Identification of another B-cell epitope in the type-specific region of equine herpesvirus 4 glycoprotein G.
作者信息
Maeda Ken, Mizukoshi Fuminori, Hamano Masataka, Kai Kazushige, Kondo Takashi, Matsumura Tomio
机构信息
Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
出版信息
Clin Diagn Lab Immunol. 2005 Jan;12(1):122-4. doi: 10.1128/CDLI.12.1.122-124.2005.
Recently, a novel 12-mer B-cell epitope, MKNNPIYSEGSL, in the type-specific region of equine herpesvirus 1 (EHV-1) glycoprotein G (gG) was identified and used as an antigen for enzyme-linked immunosorbent assay (Maeda et al., J. Clin. Microbiol. 42:1095-1098, 2004). Although our prototype strain, TH20p, possesses two repeat sequences containing the B-cell epitope, the EHV-4 NS80567 strain has two repeat sequences that are not identical. One repeat sequence stretch contained the B-cell epitope, while the other contained the 11-mer, MKNNPVYSESL (underlining indicates a different amino acid). In this study, heterogeneity of the type-specific region was compared among Japanese EHV-4 isolates. The 11-mer peptide, MKNNPVYSESL, specifically reacted with sera from horses naturally infected with EHV-4 but not with sera from horses experimentally infected with EHV-4 TH20p. The 11-mer peptide may be another B-cell epitope in the type-specific region.
最近，在马疱疹病毒1型（EHV-1）糖蛋白G（gG）的型特异性区域中鉴定出一种新的12肽B细胞表位MKNNPIYSEGSL，并将其用作酶联免疫吸附测定的抗原（前田等人，《临床微生物学杂志》42:1095 - 1098，2004年）。虽然我们的原型毒株TH20p拥有两个包含该B细胞表位的重复序列，但EHV-4 NS80567毒株有两个不同的重复序列。一个重复序列片段包含该B细胞表位，而另一个包含11肽MKNNPVYSESL（下划线表示不同的氨基酸）。在本研究中，比较了日本EHV-4分离株型特异性区域的异质性。11肽MKNNPVYSESL与自然感染EHV-4的马的血清发生特异性反应，但与实验感染EHV-4 TH20p的马的血清不发生反应。该11肽可能是型特异性区域中的另一个B细胞表位。
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