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通过使用糖蛋白G的合成24聚体重复序列作为抗原改进4型马疱疹病毒的酶联免疫吸附测定法。

Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen.

作者信息

Bannai Hiroshi, Nemoto Manabu, Tsujimura Koji, Yamanaka Takashi, Maeda Ken, Kondo Takashi

机构信息

Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.

出版信息

J Vet Med Sci. 2016 Feb;78(2):309-11. doi: 10.1292/jvms.15-0275. Epub 2015 Oct 1.

DOI:10.1292/jvms.15-0275
PMID:26424485
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4785124/
Abstract

To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%), whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer ELISA.

摘要

为提高用于检测马疱疹病毒4型（EHV-4）的酶联免疫吸附测定（ELISA）的灵敏度，该测定使用糖蛋白G的12肽（gG4-12-mer：MKNNPIYSEGSL）[4]，我们使用了由24肽重复序列组成的更长肽段（gG4-24-mer：MKNNPIYSEGSLMLNVQHDDSIHT）作为抗原。经EHV-4实验感染的马的血清对gG4-24-mer肽的反应比对gG4-12-mer肽的反应强烈得多。我们使用肽ELISA检测自然感染EHV-4的马（n = 40）的配对血清。gG4-24-mer ELISA检测到37个阳性样本（92.5%），而gG4-12-mer ELISA仅检测到28个（70.0%）。gG4-24-mer ELISA比gG4-12-mer ELISA灵敏得多。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa86/4785124/b7dfcf7f544d/jvms-78-309-g001.jpg

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J Clin Microbiol. 2004 Mar;42(3):1095-8. doi: 10.1128/JCM.42.3.1095-1098.2004.
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