Han Hyo Shim, Koh Young Jin, Hur Jae-Seoun, Jung Jae Sung
Department of Biology, Sunchon National University, Sunchon 540-742, Republic of Korea.
J Microbiol. 2004 Dec;42(4):365-8.
The occurrence of strA-strB streptomycin-resistance genes within transposon Tn5393 was examined in Pseudomonas syringae pv. actinidiae, P. syringae pv. syringae, and P. marginalis, isolated from kiwifruit plants in Korea and Japan. PCR amplification with primers specific to strA-strB revealed that three of the tested Pseudomonas species harbored these genes for a streptomycin-resistance determinant. Tn5393, containing strA-strB, was also identified with PCR primers designed to amplify parts of tnpA, res, and tnpR. No IS elements were detected within tnpR, nor were they found in the intergenic region between tnpR and strA. Nucleotide sequence analysis indicated that the strA sequence of P. syringae pv. actinidiae contained a single nucleotide alteration at position 593 (CAA-->CGA), as compared to Tn5393a in P. syringae pv. syringae. This resulted in an amino acid change, from Gln to Arg.
在从韩国和日本的猕猴桃植株中分离出的奇异假单胞菌猕猴桃致病变种、丁香假单胞菌丁香致病变种和边缘假单胞菌中，检测了转座子Tn5393内strA-strB链霉素抗性基因的存在情况。用针对strA-strB的特异性引物进行PCR扩增，结果显示，三种受试假单胞菌物种都含有这些链霉素抗性决定簇的基因。还使用设计用于扩增tnpA、res和tnpR部分的PCR引物鉴定了含有strA-strB的Tn5393。在tnpR内未检测到插入序列元件，在tnpR和strA之间的基因间隔区也未发现。核苷酸序列分析表明，与丁香假单胞菌丁香致病变种中的Tn5393a相比，奇异假单胞菌猕猴桃致病变种的strA序列在第593位有一个单核苷酸改变（CAA→CGA）。这导致了一个氨基酸变化，从谷氨酰胺变为精氨酸。
