Lee Young Sun, Kim Gyoung Hee, Koh Young Jin, Jung Jae Sung
Department of Biology, Sunchon National University, Suncheon 57922, Korea.
Department of Plant Medicine, Sunchon National University, Suncheon 57922, Korea.
Plant Pathol J. 2021 Oct;37(5):489-493. doi: 10.5423/PPJ.NT.05.2021.0078. Epub 2021 Oct 1.
Bacterial canker is a devastating disease of kiwifruit caused by the bacterium Pseudomonas syringe pv. actinidiae. Canker disease of kiwifruit in Korea has been controlled using streptomycin for more than two decades. Four streptomycin-resistant strains, belonging to biovar 2, which are found only in Korea, were collected between 2013 and 2014 from different orchards located in Jeju, Korea. The genetic background for streptomycin resistance among P. syringe pv. actinidiae strains were determined by examining the presence of strA-strB or aadA, which are genes frequently found in streptomycin-resistant bacteria, and a point mutation at codon 43 in the rpsL gene. All four streptomycin-resistant strains of P. syringe pv. actinidiae investigated in this study contained strA-strB as a resistant determinant. The presence of the aadA gene and a mutation in codon 43 of the rpsL gene was not identified.
细菌性溃疡病是由丁香假单胞菌猕猴桃致病变种引起的猕猴桃毁灭性病害。在韩国，猕猴桃溃疡病使用链霉素防治已有二十多年。2013年至2014年间，从韩国济州岛不同果园收集了4株链霉素抗性菌株，它们属于生物变种2，且仅在韩国被发现。通过检测链霉素抗性细菌中常见的strA-strB或aadA基因以及rpsL基因第43位密码子的点突变，确定了丁香假单胞菌猕猴桃致病变种菌株中链霉素抗性的遗传背景。本研究中调查的所有4株丁香假单胞菌猕猴桃致病变种链霉素抗性菌株均含有strA-strB作为抗性决定因素。未检测到aadA基因的存在以及rpsL基因第43位密码子的突变。
