Ren Ganggang, Ding Zhenghao, Pan Xin, Wei Guohai, Wang Peiyi, Liu Liwei
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals, Guizhou University, Huaxi District, Guiyang 550025, China.
School of Chemistry and Chemical Engineering, Guizhou University, Huaxi District, Guiyang 550025, China.
Antibiotics (Basel). 2022 Jul 4;11(7):891. doi: 10.3390/antibiotics11070891.
Kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae reduces kiwifruit crop yield and quality, leading to economic losses. Unfortunately, few agents for its control are available. We prepared three kinds of copper-based nanoparticles and applied them to control kiwifruit bacterial canker. The successful synthesis of Cu(OH)2 nanowires, Cu3(PO4)2 nanosheets, and Cu4(OH)6Cl2 nanoparticles were confirmed by transmission and scanning electron microscopy, energy dispersive spectroscopy, X-ray diffraction analysis, and X-ray photoelectron spectroscopy. The minimum bactericidal concentrations (MBCs) of the three nanoparticles were 1.56 μg/mL, which exceeded that of the commercial agent thiodiazole copper (MBC > 100 μg/mL). The imaging results indicate that the nanoparticles could interact with bacterial surfaces and kill bacteria by inducing reactive oxygen species’ accumulation and disrupting cell walls. The protective activities of Cu(OH)2 nanowires and Cu3(PO4)2 nanosheets were 59.8% and 63.2%, respectively, similar to thiodiazole copper (64.4%) and better than the Cu4(OH)6Cl2 nanoparticles (40.2%). The therapeutic activity of Cu4(OH)6Cl2 nanoparticles (67.1%) bested that of Cu(OH)2 nanowires (43.9%), Cu3(PO4)2 nanosheets (56.1%), and thiodiazole copper (53.7%). Their therapeutic and protective activities for control of kiwifruit bacterial canker differed in vivo, which was related to their sizes and morphologies. This study suggests these copper-based nanoparticles as alternatives to conventional bactericides for controlling kiwifruit diseases.
由丁香假单胞菌猕猴桃致病变种引起的猕猴桃细菌性溃疡病会降低猕猴桃的产量和品质，导致经济损失。不幸的是，可用的防治药剂很少。我们制备了三种铜基纳米颗粒并将其用于防治猕猴桃细菌性溃疡病。通过透射电子显微镜、扫描电子显微镜、能量色散光谱、X射线衍射分析和X射线光电子能谱证实了氢氧化铜纳米线、磷酸铜纳米片和碱式氯化铜纳米颗粒的成功合成。这三种纳米颗粒的最低杀菌浓度（MBC）均为1.56μg/mL，超过了市售药剂噻菌铜（MBC>100μg/mL）。成像结果表明，纳米颗粒可以与细菌表面相互作用，并通过诱导活性氧的积累和破坏细胞壁来杀死细菌。氢氧化铜纳米线和磷酸铜纳米片的保护活性分别为59.8%和63.2%，与噻菌铜（64.4%）相似，且优于碱式氯化铜纳米颗粒（40.2%）。碱式氯化铜纳米颗粒的治疗活性（67.1%）优于氢氧化铜纳米线（43.9%）、磷酸铜纳米片（56.1%）和噻菌铜（53.7%）。它们对猕猴桃细菌性溃疡病的治疗和保护活性在体内有所不同，这与它们的大小和形态有关。本研究表明，这些铜基纳米颗粒可作为控制猕猴桃病害的传统杀菌剂的替代品。
