[高效液相色谱法测定香附油中α-香附酮的含量]
[Determination of alpha-cyperone in Rhizoma Cyperi oil by HPLC].
作者信息
Liang Congqing, Luo Yuansheng, Zhong Zhaojian, Fu Jun, Su Chunyan
机构信息
Guangdong College of Pharmacy, Guangzhou.
出版信息
Zhong Yao Cai. 2004 Aug;27(8):578-9.
PMID:15658819
Abstract
OBJECTIVE
To establish a method deteminating alpha-cyperone in Rhizome Cyperi oil by HPLC.
METHODS
The determination was carried out by Merck Lichrocart C18 column, with mobile phase consisted of methanol-water (68: 32), and detective wavelength 241 nm.
RESULTS
The calibration curves was linear in the range of 0. 081 - 0. 81 microg/ml (r = 0.9998). The average recovery of loading sample was 99.1%, RSD =2.2%.
CONCLUSION
The method is convenient for a good resolution.
摘要
目的
建立高效液相色谱法测定香附油中α-香附酮含量的方法。
方法
采用默克Lichrocart C18柱，以甲醇-水（68:32）为流动相，检测波长为241nm进行测定。
结果
校准曲线在0.081 - 0.81μg/ml范围内呈线性（r = 0.9998）。加样平均回收率为99.1%，相对标准偏差（RSD）为2.2%。
结论
该方法简便，分离效果好。
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