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北欧和波罗的海地区起源的栽培大麦遗传多样性及亲缘关系的简单序列重复区间分析

Inter simple sequence repeat analysis of genetic diversity and relationships in cultivated barley of Nordic and Baltic origin.

作者信息

Kolodinska Brantestam Agnese, von Bothmer Roland, Dayteg Christophe, Rashal Isaak, Tuvesson Stine, Weibull Jens

机构信息

Swedish University of Agricultural Sciences, Department of Crop Science, SE-230-53 Alnarp, Sweden.

出版信息

Hereditas. 2004;141(2):186-92. doi: 10.1111/j.1601-5223.2004.01867.x.

DOI:10.1111/j.1601-5223.2004.01867.x
PMID:15660979
Abstract

This study evaluates putative changes of genetic diversity and relationships of barley in the Nordic and Baltic countries that might have taken place during the last century as a result of commercial breeding. Four ISSR primers were used to analyse 227 accessions, yielding a total of 47 polymorphic loci. Shannon-Weaver diversity values for each locus ranged from 0.012 to 0.693. Overall, there were no significant changes of genetic diversity observed over time. A significant decrease of diversity was, however, observed in material from the southern parts of the Nordic and Baltic countries. In material from the northern parts no decrease of diversity was observed. The genetic diversity of six-rowed barley bred in the middle of the 20th century was low, but there was no significant difference between modern accessions and landraces or old cultivars. The magnitude in changes of genetic diversity differed also in material from different countries of origin. A cluster analysis clearly separated the material into two groups. The first cluster included 86.5% of all six-rowed accessions, whereas the second cluster contained 97.4% of all two-rowed accessions.

摘要

本研究评估了北欧和波罗的海国家大麦的遗传多样性和亲缘关系可能在上个世纪因商业育种而发生的假定变化。使用4种ISSR引物分析了227份材料,共产生47个多态性位点。每个位点的香农-韦弗多样性值在0.012至0.693之间。总体而言,未观察到遗传多样性随时间有显著变化。然而,在北欧和波罗的海国家南部的材料中观察到多样性显著下降。在北部的材料中未观察到多样性下降。20世纪中叶培育的六棱大麦的遗传多样性较低,但现代材料与地方品种或老品种之间没有显著差异。来自不同原产国的材料中遗传多样性的变化幅度也有所不同。聚类分析清楚地将材料分为两组。第一组包含所有六棱材料的86.5%,而第二组包含所有二棱材料的97.4%。

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