Xing Ronge, Yu Huahua, Liu Song, Zhang Weiwei, Zhang Quanbin, Li Zhien, Li Pengcheng
Institute of Oceanology, The Chinese Academy of Sciences, Qingdao 266071,China.
Bioorg Med Chem. 2005 Feb 15;13(4):1387-92. doi: 10.1016/j.bmc.2004.11.002.
Differently regioselective chitosan sulfates were prepared according to Hanno Baumann's methods. Their antioxidant potencies were investigated employing various established in vitro systems, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH)/superoxide/hydroxyl radicals scavenging, reducing power, iron ion chelating and total antioxidant activity. All kinds of sulfated chitosans (HCTS, TSCTS, SCTS, TCTS) showed strong inhibitory activity toward superoxide radical by the PMS-NADH system compared to Vc. According to the above-mentioned order their IC50 were 0.012, 0.040, 0.015, 0.022 mg/mL, respectively, however, scavenging activity of Vc on superoxide radical was 68.19% at 2.0 mg/mL. Scavenging activity of superoxide radical was found to be in the order of HCTS>SCTS>TCTS>TSCTS>Vc. Furthermore, all kinds of sulfated chitosans exhibited strong concentration-dependent inhibition of deoxyribose oxidation. Except for HCTS, others had stronger scavenging activity on hydroxyl radical than Vc. Scavenging effect of TSCTS on 1,1-diphenyl-2-picrylhydrazyl radical was little lower than that of BHA, but better than that of others. All kinds of sulfated chitosans were efficient in the reducing power, especially TSCTS. TSCTS and TCTS showed considerable ferrous ion chelating potency. The data obtained in vitro models clearly establish the antioxidant potency of all kinds of sulfated chitosans. These in vitro results suggested the possibility that sulfated chitosans could be effectively employed as ingredient in health or functional food, to alleviate oxidative stress. However, comprehensive studies need to be conducted to ascertain the in vivo safety of sulfated chitosans in experimental animal models.
按照汉诺·鲍曼的方法制备了不同区域选择性的壳聚糖硫酸盐。采用各种成熟的体外系统研究了它们的抗氧化能力，如1,1-二苯基-2-苦基肼（DPPH）/超氧阴离子/羟基自由基清除、还原能力、铁离子螯合和总抗氧化活性。与维生素C相比，各种硫酸化壳聚糖（HCTS、TSCTS、SCTS、TCTS）通过PMS-NADH系统对超氧阴离子自由基表现出较强的抑制活性。按照上述顺序，它们的IC50分别为0.012、0.040、0.015、0.022 mg/mL，然而，维生素C在2.0 mg/mL时对超氧阴离子自由基的清除活性为68.19%。发现超氧阴离子自由基的清除活性顺序为HCTS>SCTS>TCTS>TSCTS>维生素C。此外，各种硫酸化壳聚糖对脱氧核糖氧化表现出较强的浓度依赖性抑制作用。除HCTS外，其他对羟基自由基的清除活性均强于维生素C。TSCTS对1,1-二苯基-2-苦基肼自由基的清除效果略低于丁基羟基茴香醚（BHA），但优于其他物质。各种硫酸化壳聚糖的还原能力都很强，尤其是TSCTS。TSCTS和TCTS表现出相当可观的亚铁离子螯合能力。体外模型获得的数据清楚地证实了各种硫酸化壳聚糖的抗氧化能力。这些体外结果表明，硫酸化壳聚糖有可能有效地用作健康或功能性食品的成分，以减轻氧化应激。然而，需要进行全面研究以确定硫酸化壳聚糖在实验动物模型中的体内安全性。
