俄罗斯西西伯利亚全沟硬蜱中亨氏巴尔通体的半巢式PCR检测
Semi-nested PCR detection of Bartonella henselae in Ixodes persulcatus ticks from Western Siberia, Russia.
作者信息
Morozova O V, Cabello F C, Dobrotvorsky A K
机构信息
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia.
出版信息
Vector Borne Zoonotic Dis. 2004 Winter;4(4):306-9. doi: 10.1089/vbz.2004.4.306.
Questing adult Ixodes persulcatus ticks from Western Siberia, Russia were tested for infections with Bartonella spp. using seminested PCR assay with primers specific to the groEL gene. The proportion of ticks infected with Bartonella spp. was 44% in 2002 (n = 50) and 38% in 2003 (n = 50). Nucleotide sequences of a portion of the PCR products corresponded to Bartonella henselae species.
对从俄罗斯西西伯利亚采集的成年全沟硬蜱进行检测，使用针对groEL基因的引物通过半巢式PCR检测法检测其是否感染巴尔通体属细菌。2002年感染巴尔通体属细菌的蜱的比例为44%（n = 50），2003年为38%（n = 50）。部分PCR产物的核苷酸序列与亨氏巴尔通体种相对应。
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