Moalli Pamela A, Howden Nancy S, Lowder Jerry L, Navarro Jennifer, Debes Kristen M, Abramowitch Steven D, Woo Savio L-Y
Magee-Womens Research Institute and the Department of Obstetrics and Gynecology at Magee Womens Hospital, Pittsburgh, PA 15213, USA.
Am J Obstet Gynecol. 2005 Jan;192(1):80-8. doi: 10.1016/j.ajog.2004.07.008.
The purpose of this study was to investigate the rat as a model for evaluating the structural properties of the vagina and its supportive tissues.
The in situ relationships between the vagina and its supportive tissues were studied grossly (n = 10), and in serial cross sections (n = 4). For biomechanical testing (n = 10), the lumbar spine was fixed to a testing machine while the distal vagina was gripped with a soft-tissue clamp mounted to a load-cell on the crosshead of the machine. The vagina was systematically pulled through the pelvis until failure of the supportive tissues occurred. Parameters describing the structural properties of the tissues (linear stiffness, ultimate load, ultimate elongation, and energy absorbed to failure) were determined from the resulting load-elongation curve. A coefficient of correlation (R) was used to determine experimental reproducibility.
The rat vagina is supported by structures analogous to those in humans. The parameters describing the structural properties of these tissues were highly correlated among specimens (R = 0.95). The mechanism of failure was similar for all specimens.
We have developed a mechanical testing protocol in which the structural properties of the vagina-supportive tissue complex can be measured. This will be a powerful tool for testing the impact of life events on vaginal support.
本研究旨在探究大鼠作为评估阴道及其支持组织结构特性模型的可行性。
对阴道及其支持组织的原位关系进行大体观察（n = 10）和连续横断面观察（n = 4）。对于生物力学测试（n = 10），将腰椎固定于测试机上，同时用安装在测试机十字头上的测力传感器的软组织夹夹住阴道远端。系统地牵拉阴道通过骨盆，直至支持组织发生断裂。从所得的载荷-伸长曲线确定描述组织结构特性的参数（线性刚度、极限载荷、极限伸长和断裂吸收能量）。使用相关系数（R）确定实验的可重复性。
大鼠阴道由与人类类似的结构支持。描述这些组织结构特性的参数在各标本间高度相关（R = 0.95）。所有标本的断裂机制相似。
我们已开发出一种力学测试方案，可测量阴道-支持组织复合体的结构特性。这将成为测试生活事件对阴道支持影响的有力工具。
