Rubod Chrystèle, Boukerrou Malik, Brieu Mathias, Dubois Patrick, Cosson Michel
Clinique de Chirurgie Gynécologique, Hôpital Jeanne de Flandre, Lille, and Universitaire de Lille, France.
J Urol. 2007 Jul;178(1):320-5; discussion 325. doi: 10.1016/j.juro.2007.03.040. Epub 2007 May 17.
We established a reliable experimental protocol to characterize the biomechanical properties of vaginal tissue and guarantee good test repeatability.
Because of the large quantities of tissue required to establish the protocol, we worked on ewes according to animal ethics laws. To study the mechanical properties of ewe vaginal tissue we used unidirectional tension tests at a constant deformation rate. Rupture tests were performed under different experimental conditions to analyze the influence of each condition.
Tissue underwent exhaustive tests. The parameters studied were sampling, freezing, preservation conditions, hygrometry and temperature during vaginal tissue tests, and the rate of deformation during tests. As previously noted, vaginal tissue is anisotropic and the collection has been tested previously. We noted that freezing tissue had no consequences on the mechanical response of tissue during unidirectional tension testing. The experimental conditions that we defined (temperature, hygrometry and rate of deformation) allowed us to have reproducible tests.
Results and analyses allowed us to determine the best reference protocol.
我们建立了一个可靠的实验方案，以表征阴道组织的生物力学特性并确保良好的测试重复性。
由于建立该方案需要大量组织，我们根据动物伦理法对母羊进行研究。为了研究母羊阴道组织的力学特性，我们在恒定变形速率下进行单向拉伸试验。在不同实验条件下进行破裂试验，以分析每种条件的影响。
组织进行了详尽的测试。研究的参数包括采样、冷冻、保存条件、阴道组织测试期间的湿度和温度，以及测试期间的变形速率。如前所述，阴道组织是各向异性的，且该采集样本之前已进行过测试。我们注意到，在单向拉伸试验期间，冷冻组织对组织的力学响应没有影响。我们定义的实验条件（温度、湿度和变形速率）使我们能够进行可重复的测试。
结果和分析使我们能够确定最佳参考方案。
