外周血中CD4(+)IL-13(+)细胞与儿童特应性皮炎的严重程度密切相关。

CD4(+)IL-13(+) cells in peripheral blood well correlates with the severity of atopic dermatitis in children.

作者信息

La Grutta S, Richiusa P, Pizzolanti G, Mattina A, Pajno G B, Citarrella R, Passalacqua G, Giordano C

机构信息

Allergy Unit, Children Hospital -- ARNAS, Palermo, Italy.

出版信息

Allergy. 2005 Mar;60(3):391-5. doi: 10.1111/j.1398-9995.2005.00733.x.

DOI:10.1111/j.1398-9995.2005.00733.x

PMID:15679728

Abstract

BACKGROUND

In atopic dermatitis (AD) a Th1/Th2 imbalance has been reported, and interleukin (IL)-13 seems to play a pivotal role in the inflammatory network. We tried to assess the correlation between the immunological marker CD4(+)IL-13(+) and the clinical phase of extrinsic AD in children.

METHODS

Twenty children with AD were studied. Assessed parameters were: clinical severity (SCORAD index), total serum immunoglobulin E (IgE), blood eosinophil count, and percentage of CD4(+)IFNgamma(+), CD4(+)IL-4(+), CD4(+)IL-13(+) T cells. Determinations were carried out in the acute phase and after clinical remission were achieved. Ten nonatopic-matched children served as controls.

RESULTS

At baseline, AD was mild in 25%, moderate in 50% and severe in 25% of children. In the acute phase a significant relationship between the eosinophil count and the SCORAD index was found (P = 0.0001). Blood CD4(+)IL-4(+) were significantly higher in the AD group (median 17.0, range: 13.7-21.4) than in controls (12.6, 6.4-17.2, P < 0.0001). CD4(+)IL-13(+) cells in the AD group well correlated (P = 0.0007) with SCORAD index. At remission, a significant correlation between SCORAD index and eosinophil count was found (P < 0.03) and the percentage of CD4(+)IL-13(+) cells globally decreased (P < 0.0001), while no difference was found among SCORAD classes.

CONCLUSION

This study confirms the Th2 profile predominance in the peripheral blood of children with AD, and evidences close relationship between the number of CD4(+)IL-13(+) T cells and the disease's severity.

摘要

背景

在特应性皮炎（AD）中，已有报道存在Th1/Th2失衡，且白细胞介素（IL）-13似乎在炎症网络中起关键作用。我们试图评估免疫标志物CD4(+)IL-13(+)与儿童外源性AD临床阶段之间的相关性。

方法

对20例AD患儿进行研究。评估参数包括：临床严重程度（SCORAD指数）、血清总免疫球蛋白E（IgE）、血液嗜酸性粒细胞计数以及CD4(+)IFNγ(+)、CD4(+)IL-4(+)、CD4(+)IL-13(+) T细胞的百分比。在急性期和临床缓解后进行测定。10例非特应性匹配儿童作为对照。

结果

基线时，25%的患儿AD病情轻度，50%为中度，25%为重度。在急性期，发现嗜酸性粒细胞计数与SCORAD指数之间存在显著相关性（P = 0.0001）。AD组血液中CD4(+)IL-4(+)显著高于对照组（中位数17.0，范围：13.7 - 21.4）（对照组为12.6，6.4 - 17.2，P < 0.0001）。AD组中CD4(+)IL-13(+)细胞与SCORAD指数密切相关（P = 0.0007）。缓解期，发现SCORAD指数与嗜酸性粒细胞计数之间存在显著相关性（P < 0.03），且CD4(+)IL-13(+)细胞的百分比总体下降（P < 0.0001），而SCORAD分级之间未发现差异。