一种用于定量脑脊液中神经丝轻链的酶免疫测定法。
An enzyme immunoassay to quantify neurofilament light chain in cerebrospinal fluid.
作者信息
Van Geel W J A, Rosengren L E, Verbeek M M
机构信息
Laboratory of Pediatrics and Neurology, University Medical Centre Nijmegen, The Netherlands.
出版信息
J Immunol Methods. 2005 Jan;296(1-2):179-85. doi: 10.1016/j.jim.2004.11.015. Epub 2004 Dec 9.
Neurofilament light chain is a component of the axonal cytoskeleton. The concentration of the neurofilament light chain in cerebrospinal fluid may reflect axonal damage or the extent of white matter damage. In this study we describe a sensitive immunoassay for the detection of neurofilament light chain in cerebrospinal fluid using commercially available materials. The detection limit of the assay was 5 ng/l and the assay was linear up to 390 ng/l. Mean recovery was 91.5% and inter-assay and intra-assay coefficients of variation were below 18%. Strongly increased levels of neurofilament light chain were observed in patients with cerebrovascular accidents, subarachnoid hemorrhage and severe traumatic brain injury, suggesting the occurrence of axonal damage in these conditions.
神经丝轻链是轴突细胞骨架的一个组成部分。脑脊液中神经丝轻链的浓度可能反映轴突损伤或白质损伤的程度。在本研究中，我们描述了一种使用市售材料检测脑脊液中神经丝轻链的灵敏免疫测定法。该测定法的检测限为5 ng/l，在高达390 ng/l的范围内呈线性。平均回收率为91.5%，批间和批内变异系数均低于18%。在脑血管意外、蛛网膜下腔出血和重度创伤性脑损伤患者中观察到神经丝轻链水平显著升高，提示在这些情况下发生了轴突损伤。
Zotero 插件